沙立度胺抑制乳腺癌细胞增殖的作用机制  

Antiproliferation Effect and Its Mechanism of Thalidomide on Breast Cancer Cells in vitro

作　　者：李书芹[1] 季艳霞[2] 杨志勇[2] 李海燕[2] 张振军[2] 贾军刚[2] 万良刚[2] 

机构地区：[1]河北省邯郸市中心医院血液科,056001 [2]河北省邯郸市中心医院放疗科,056001

出　　处：《肿瘤防治研究》2008年第8期560-562,578,共4页Cancer Research on Prevention and Treatment

基　　金：河北省科技攻关计划项目(06271401)

摘　　要：目的研究沙立度胺抑制乳腺癌细胞增殖的机制。方法流式细胞仪检测沙立度胺对细胞凋亡和细胞周期的影响;半定量RT-PCR方法观察沙立度胺对MCF-7和MDA-MB-231细胞VEGF mR-NA表达的影响。结果沙立度胺作用后的乳腺癌细胞在流式细胞分析的DNA直方图上显现细胞凋亡峰(亚G1期);沙立度胺抑制两株细胞VEGF mRNA的表达。结论沙立度胺可能通过诱导MCF-7和MDA-MB-231细胞凋亡以及抑制细胞VEGF mRNA表达从而抑制MCF-7和MDA-MB-231细胞增殖。Objective To investigate the antiproliferation effect and its mechanism of thalidomide on breast cancer cells in vitro. Methods （1） The influence of thalidomide on apoptotisis and cell cycle were detected in treated cells by FCM assay. （2）The effect of thalidomide on expression of VEGF mRNA in human breast cancer cell lines was determined by reverse transcriptase-polymerase chain reaction （RT-PCR） method. Results （1）Apoptotic cells were found in treated cells, the breast cancer cell presented the seeond period of G1 in the DNA histogram of FCM. （2）Thalidomide inhibited the level of VEGF mRNA expression. Conclusion The inducement apoptotic and inhibition the level of VEGF mRNA expression were the mechanisms of thalidomide on antiproliferation in breast cancer cell lines.

关 键 词：乳腺肿瘤 细胞系 沙立度胺 凋亡 内皮生长因子 

分 类 号：R737.9[医药卫生—肿瘤]