白细胞介素15及其受体在儿童急性白血病中的表达和作用探讨  

Levels of Interleukin-15 and Its Receptor Subunit Expression in Children Acute Leukemia

作　　者：叶中绿[1] 陈铭珍[1] 陈日玲[1] 黄秀兰[1] 庞伟君[2] 陈水文 

机构地区：[1]广东医学院附属医院儿科,广东湛江524001 [2]广东医学院附属医院检验科,广东湛江524001 [3]深圳市保安区妇幼保健院儿科

出　　处：《肿瘤防治研究》2008年第8期579-582,共4页Cancer Research on Prevention and Treatment

基　　金：广东省自然科学基金资助项目(5011591)

摘　　要：目的探讨IL-15水平及其受体在儿童急性白血病中的表达及作用。方法取确诊未治的儿童急性淋巴细胞白血病(ALL)、儿童急性非淋巴细胞白血病(ANLL)和非白血病组儿童各20例,20例正常儿童的血清,测定不同组别对象血清IL-15的水平和不同组别对象的骨髓单个核细胞IL-15受体三个亚基α、β、γ的表达并进行半定量分析。结果急性白血病患儿血清IL-15水平较正常和对照组均出现明显下降;IL-15受体α、β、γ亚基的表达水平也均较正常组和对照组明显下降,但ALL与ANLL组之间和非白血病组与对照组之间差异无统计学意义。结论急性白血病患儿无论血清IL-15水平及其骨髓单个核细胞IL-15受体α、β、γ均出现下降。Objective To investigate the expression of IL-15 and its receptor in children acute leukemia, contribute to understand their function on acute leukemia. Methods Every group of acute lymphocytic leukemia（ALL） ,acute myeloid leukemia （ANLL） or non-leukemia group had 20 children who did not receive any treatment. The expression of the interleukin-15 receptor alpha, beta, gamma mRNA in bone marrow were examined by RT-PCR, and make an Semiquantitative analysis. The level of IL-15 in serum of every group by means of ELISA. Results The level of IL-15 in AL（ALL and ANLL）group, were significant was assayed in statistics（P〈0. 05）with healthy children and non-leukemia group. The difference which the IL-15 level of ALL compared with ANLL or the level of healthy children compared with non- leukemia group is neglected in statistics（P〉0. 05）. The expression of IL-15R alpha, beta,gamma mRNA in AL is significantly different than that of healthy and non-leukemia children. The difference which the IL-15 alpha,beta,gamma of ALL compared with ANLL or the level of healthy children compared with non-leukemia group is neglectable in statistics （P〉0. 05）. Conclusion The IL-15 level of AL children is lower than control group. And the expression that of IL-15Rα, β, γ subunit in bone marrow mononuclear cell of AL is smaller than those of non-leukemia group.

关 键 词：急性白血病 白细胞介素15 受体 基因 

分 类 号：R733.7[医药卫生—肿瘤]