胰腺癌中CXCL12-CXCR4生物学轴与VEGF-C的表达及相关性研究  

Correlation analysis of CXCL12,CXCR4 and VEGF-C expression and clinical significance in pancreatic cancer

作　　者：张红雨[1] 费立明[2] 王长亮[3] 赵文华[3] 张波[3] 周武元[3] 于文胜[3] 仲伟霞[3] 李胜[3] 

机构地区：[1]山东大学齐鲁医院老年病科,山东济南250012 [2]烟台市烟台山医院普外科,山东烟台264001 [3]山东省肿瘤防治研究院肝胆外科,山东济南250117

出　　处：《中国现代普通外科进展》2008年第4期283-286,共4页Chinese Journal of Current Advances in General Surgery

基　　金：国家自然科学基金资助项目(30600603);山东省自然科学基金资助项目(Y2007F06);山东省科技发展计划资助项目(20070GG20002022)

摘　　要：目的:研究胰腺癌中CXCL12-CXCR4生物学轴(CXCL12-CXCR4 biological axis)与血管内皮生长因子-C(vascular endothelial growth factor-C,VEGF-C)的表达情况,探讨其表达与胰腺癌的临床病理关系。方法:应用免疫组织化学和RT-PCR技术检测30例胰腺癌、癌旁、正常胰腺和胰周淋巴结组织中CXCL12、CXCR4和VEGF-C的表达情况。结果:CXCL12蛋白在胰腺癌组织中呈低表达状态,在正常胰腺组织、癌旁组织和胰周淋巴结组织均呈中等表达状态(P<0.01),RT-PCR结果也证实此结果,与胰腺癌组织中的表达相比较差异均有统计学意义(P<0.01)。CXCR4蛋白在胰腺癌组织、癌旁组织和胰周淋巴结均呈高表达状态,在正常胰腺组织中呈低表达状态,RT-PCR结果同样证实。经Spearman检验,胰腺癌组织中CXCR4与VEGF-C蛋白的表达具有显著的相关性(P=0.038)。结论:CXCR4的表达与胰腺癌淋巴结转移密切相关,其高表达对胰腺癌中微淋巴管的生成可能起促进作用。胰腺癌组织中CXCR4与VEGF-C蛋白的表达具有显著的相关性,两者可能对胰腺癌的淋巴管生成和淋巴结转移起协同作用。Objectivet To investigate the expression of CXCL12-CXCR4 and VEGF-C in pancreatic cancer and relation to clinical pathology. Methods The tissue samples including PAC, the cancerous peripheral tissues, the normal pancreatic tissues and peripheral lympho nodes were obtained from 30 patients with PAC. The expressions of CXCL12, CXCR4and VEGF-C proteins in these tissues were assayed by immunohistochernical staining. The expressions of CXCL12, CXCR4 and VEGF-C mRNA in PAC were also investigated by fluorescence quantitative real-time PCR. Results： In all the samples, the positive rates of CXCL12 protein in PAC, the cancerous peripheral tissues, the normal pancreatic tissues and peripheral lympho nodes were respectively 13.3% （4/30）, 46.7% （14/30）,56.7% （17/30） and 50.0% （15/30）. The positive rates of CXCR4 protein in PAC, the cancerous peripheral tissues, the normal pancreatic tissues and peripheral lympho nodes were respectively 80.0% （24/30）, 70.0% （21/30）, 26.7%（8/30） and 73.3% （22/30）. The expression levels of CXCR4 mRNA in PAC tissues, the cancerous peripheral tissues and peripheral lympho nodes were higher than that in the normal pancreatic tissues （P〈0.01）. There was significant correlation between the expressions of CXCR4 and VEGF-C protein in PAC which was tested by Spearman correlation test （P=0.038）. Conclusion： The expression of CXCR4 is significantly associated with lymphatic metastasis of PAC.There is significant correlation between the expressions of CXCR4 and VEGF-C protein in PAC. CXCR4 and VEGF-C maybe promote the development of PAC mutually.

关 键 词：胰腺肿瘤 CXCL12 CXCR4 血管内皮生长因子 

分 类 号：R736.9[医药卫生—肿瘤]