电针不同穴组对胃癌大鼠术后免疫功能的影响  

Effects of Electroacupuncture of "Zusanli" (ST 36),"Hegu" (LI 4) and/or "Sanyinjiao" (SP 9) on Immunofunction in Gastric Carcinectomy Rats

作　　者：赖敏[1] 王淑美[1] 汪莹[1] 唐成林[1] 孔令文 徐晓玉[3] 

机构地区：[1]重庆医科大学中医药学院,重庆401331 [2]重庆市急救中心,重庆400001 [3]西南大学药学院与中医药学院,重庆400715

出　　处：《针刺研究》2008年第4期245-249,共5页Acupuncture Research

基　　金：重庆市教委资助课题[渝科教(2006)8号]

摘　　要：目的：研究针刺“足三里”“合谷”“三阴交”3个穴位不同配穴组对胃癌大鼠术后免疫功能的影响。方法：雄性Wistar大鼠用Walker-256细胞株复制种植性胃癌模型，并行手术根治。术后第3天，将大鼠随机分为9组：足三里组、合谷组、三阴交组、足三里＋合谷组、合谷＋三阴交组、足三里＋三阴交组、足三里＋合谷＋三阴交组、非穴位针刺组和模型组，每组6只；另设正常组6只。电针治疗选择疏密波，频率2～100Hz，电流强度1～3mA，每日1次，持续30min，共7d。用单向免疫扩散法测定各组大鼠外周血中体液免疫指标：免疫球蛋白IgG、IgM、IgA，补体C3、C4含量；流式细胞仪测定细胞免疫指标：T淋巴细胞亚群CD4^＋、CD8^＋。细胞百分率及CD4^＋／CD8^＋比值。结果：与模型组比较，非穴位针刺组IgG、IgM、IgA、C3、C4、CD4^＋、CD8^＋。及CD4^＋／CD^＋比值差异无显著性意义（P〉0．05）；针刺各组，其体液免疫、细胞免疫水平增高，与模型组比较差异有显著性（P〈0．05，0．01）。与正常组比较，足三里＋合谷＋三阴交组体液免疫、细胞免疫水平差异无显著性意义（P〉0．05）。结论：电针“足三里”“合谷”“三阴交”对大鼠胃癌根治术后低下的免疫功能有明显促进作用，3个穴位同时使用刺激机体免疫功能作用最好。Objective To study the effect of electroacupuncture （EA） of ＂Zusanli＂ （ST 36）, ＂Hegu＂ （LI 4） and/or ＂Sanyinjiao＂ （SP 6） on immune functions in gastric carcinoma rats after operation. Methods Wistar rats were randomly divided into normal control, ST36, LI4, SP6, ST36 ＋ LI4, ST36 ＋ SP6, LI4 ＋ SP6, ST36 ＋ LI4 ＋ SP6, non-acupoint （about 10 mm lateral to ST36） and model groups, with 6 cases in each. Gastric carcinoma model was made by intraperitoneal injection of Walker-256 cloned strain （0. 1 ml, 2× 10^7 cells）. EA （2- 100 Hz, 1 -3 mA） was applied to these acupoints for 30 min, once daily for 7 days. Serum IgG, IgM, IgA, 03 and 04 contents were detected with simple immunodiffusion method; and CD4^＋ and CD8^＋ levels were measured by flow cytometry. Results In comparison with normal control group, serum IgG, IgM, IgA , 03, 04 and CD8^＋ contents, and CD4^＋/CD8^＋ in model group decreased significantly （ P 〈0.05, 0, 01 ） ; while CD8^＋ content in model group increased remarkably （P〈0.01）. Compared with model group, serum IgG, IgM, IgA , 03, 04, CD4^＋, CD8^＋ and CD4^＋/CD8^＋ in non-acupoint group had no significant changes （P〉0.05）, while most of these indexes in EA groups （ST36, LI4, SP6, ST36 ＋ LI4, ST36 ＋ SP6, LI4 ＋ SP6, ST36 ＋ LI4 ＋ SP6） increased considerably （ P〈0.05, 0.01 ） except CD8＋ level （decreased significantly, P〈0.05, 0.01）. No significant differences were found among 7 EA groups （P〉0, 05）, but the effects of ST36 ＋ LI4 ＋ SP6 group were slightly better than those of the other 6 EA groups, Conclusion EA of ＂Zusanli＂ （ST 36）, ＂Hegu＂ （LI 4） or ＂Sanyinjiao＂ （SP 6） or two of them or these 3 acupoints can obviously enhance the immune function of post-surgery rats with gastric carcinoma.

关 键 词：WALKER-256 胃癌 电针 免疫功能 

分 类 号：R224.2[医药卫生—中医基础理论]