尼美舒利对A549细胞株放疗增敏作用的观察  

The investigation to the effect of radiosensitive enhancement of nimesulide in lung cancer cell line

作　　者：宋琳[1] 李少林[1] 刘芳欣[1] 王树斌[1] 柏玮[1] 彭志平[1] 

机构地区：[1]重庆医科大学基础医学院放射医学教研室,重庆400016

出　　处：《重庆医科大学学报》2008年第7期773-776,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金(30070230)

摘　　要：目的:观察尼美舒利(Nimesulide)作为环氧化酶2(Cyclooxygenase-2,COX-2)抑制剂对肺腺癌细胞株A549的放射增敏作用并探讨其作用机制。方法:对数期生长的A549细胞株,按照实验要求分为对照组、药物组、照射组及实验组,流式细胞仪检测各组肿瘤细胞凋亡率及对Bcl-2、Bax蛋白表达的影响;克隆形成实验检测放射增敏作用。结果:小剂量(75mmol/L)尼美舒利与射线照射联合应用能够上调Bax的蛋白表达,同时抑制Bcl-2蛋白表达,明显增加肿瘤细胞的凋亡,具有统计学差别。结论:COX-2抑制剂尼美舒利对人肺腺癌A549细胞株具有明显的放疗增敏作用。Objective：To investigate the enhancement of radiosensition of COX-2 inhibitor Nimesulide on lung cancer cell line （A549） in vitro and try to reaserch the possible radiosensitizing mechanisms. Methods：A549 cell line which is in logarithmic phase was derided into four group：control group,drug group,radiation group and case group. The apoptosis and the expression in protein of Bcl-2 and Bax were measured by flow cytometry（FCM）. The enhancement of radiosensitising effect was detect by clone formation experiment. Results： Low dose of Nimesulide combined with X-ray can induce the up-regulation of the protein of Bax and inhibit the expression of the protein of Bcl-2,that increased the apoptosis of tumour cell. The difference is significant. Conclusion：It is suggested that Cox-2 inhibitor- Nimesulide enhance the radiosensitivity to the cell line A549 of adenocarcinoma of lung.

关 键 词：环氧化酶2选择性抑制剂 放射敏感性 肺腺癌 凋亡 X线 

分 类 号：R730.55[医药卫生—肿瘤]