健胃愈疡片对胃十二指肠粘膜SP和ITF的表达及其抗PU复发的机理探讨  

Observance on the expressions of spasmolytic polypeptide(SP) and intestinal trefoil factors(ITF) of gastric mucosa affected by Jianweiyuyang(JWYY) pills and study of the potential mechanisim of JWYY against the recurrence of peptic ulcer

作　　者：黄国栋[1] 杨治芳[1] 游宇[1] 徐龙[1] 黄媛华[2] 唐丽君[3] 

机构地区：[1]南昌大学第一附属医院中西医结合科,南昌330006 [2]湖南中医药大学附属衡阳医院,衡阳421001 [3]湖南省人民医院,长沙410000

出　　处：《重庆医科大学学报》2008年第7期821-824,827,共5页Journal of Chongqing Medical University

摘　　要：目的:观察健胃愈疡片(Jianweiyuyang,JWYY)对溃疡粘膜解痉多肽(Spasmolytic polypeptide,SP)和肠三叶因子(Intestinal trefoil factor,ITF)表达的变化,并分析其可能的作用机制。方法:选取经胃镜证实的消化性溃疡(Peptic ulcer,PU)患者72例,其中经胃镜和组织学及尿素酶检查证实幽门螺杆菌(Helicobacter pylori,Hp)阳性的PU且中医辨证属肝郁脾虚型的患者50例。按随机分为3组,其中健胃愈疡片治疗组和雷尼替丁(Ranitidine,Rani)对照组各36例,正常对照组12例。胃粘膜组织用免疫组化检测SP的表达,Western blotting检测ITF表达的变化,并同时检测氨基己糖及磷脂含量。结果:JWYY组氨基己糖,磷脂含量增高,与雷尼替丁组比较差异有显著性(P<0.01)。免疫组化,Western blotting检测显示:PU患者氨基己糖,磷脂含量和ITF、SP的表达有直线相关关系(P<0.01)。结论:JWYY片通过提高SP和ITF的表达,及通过影响胃粘膜氨基己糖及磷脂含量,从而影响胃粘膜疏水性,加强粘液凝胶层稳定性,最终防止溃疡的产生和复发,这可能是其促进溃疡愈合的主要作用机制之一。Objective：To observe the effects of Jianweiyuyang（JWYY） pills on expressions of spasmolytic Polypeptide（SP） and intestinal trefoil factors（ITF） and analyze its potential mechanism. Methods：A Total of 76 gastric ulcer patients（final diagnosis hy gastroscope）,were randomly divided into two groups,JWYY treatment group, （36 cases,treatment group）and Ranitidine control group（36 samples,control group ）.Among them 50 Hp-positive PU patients were confirmed by gastroscope, histological inspection, and urease test,and showed the differentiation of symptoms and signs of the traditional Chinese medicine is the stagnation of liver-QI with deficiency of the spleen type for these 50 patients. There were 12 case in Normal groups. Detection on Biospy Specimens of gastric mucosa was done.The change of SP expression was detected by immunohischemicity, ITF by Western hlotting and the contents of aninohexose and phosphatide in ulcerated gastric mucosa were measured. Results： The contents of aninohexose and phosphatide of PU patients increased in JWYY group and there was significant difference between JWYY group and Ranitidine group（P〈0.01 ）. The immunohischemicity and Western blotting method showed that there has a Linear correlation between the the contents of phosphatide and aninohexose and the expressions of SP and ITF in PU patients（P〈0.01）. Conclusion：JWYY pills can prevent the occurrence and relapes of ulcer,affect the hydrophobicity of gastric mucosa and strengthen the stability of myxogellayer by elevating the expressions of ITF and SP,and effecting the contents of phosphatide and aninohexose. This is probably one of the main mechanisms of JWYY to heal ulcer quickly.

关 键 词：健胃愈疡片 消化性溃疡 复发 胃粘膜疏水性 氨基己糖 磷脂 SP ITF 

分 类 号：R286.5[医药卫生—中药学]