星形细胞瘤p16与Rb蛋白的表达及其相关性研究  

Relationship between p16 and Rb protein expression in astrocytomas

作　　者：蔺玉昌[1,2,2] 黄长铭[1,2,2] 刘道坤[1,2,2] 陈莉[1,2,2] 

机构地区：[1]南通医学院附属医院脑外科 [2]无锡市第二人民医院脑外科

出　　处：《中华病理学杂志》1997年第6期340-342,共3页Chinese Journal of Pathology

摘　　要：目的检测ｐ１６与Ｒｂ蛋白在原发性星形细胞瘤中的存在状况，以探讨不同病理类型星形细胞瘤中ｐ１６与Ｒｂ蛋白表达及其相关性。方法使用抗ｐ１６及Ｒｂ蛋白抗体对１０２例星形细胞瘤手术标本进行免疫细胞化学检测。结果低度恶性星形细胞瘤（ＷＨＯⅠ～Ⅱ级）中ｐ１６及Ｒｂ蛋白均为阳性表达，在高度恶性星形细胞瘤（ＷＨＯⅢ～Ⅳ级）中其阳性表达分别为４８．１％（２６／５４）和５７．４％（３１／５４）。在３１例Ｒｂ蛋白阳性标本中，有２４例（７７．４％）显示ｐ１６蛋白表达缺失或低表达，而在２３例Ｒｂ蛋白阴性标本中却有１９例（８２．６％）显示ｐ１６蛋白阳性或强阳性。结论（１）ｐ１６及Ｒｂ蛋白参与星形细胞瘤细胞增殖过程并影响其细胞分化；（２）ｐ１６与Ｒｂ蛋白之间阳性表达的相互抑制，可能是高度恶性星形细胞瘤的标志之一。Objective p16 and Rb protein were examined in primary astrocytomas in order to study the correlation between p16 and Rb proteins. Methods p16 and Rb pretoin were immunostained by SP immunohistochemical method in the sections of formalin fixed paraffin embedded tumor tissue from 102 patients with astrocytoma brain tumors. Results p16 and Rb protein were expressed in all low grade (WHO Grade Ⅰ and Ⅱ) astrocytomas but only in 48.1% and 57.4% astrocytomas of Grade Ⅲ and Grade Ⅳ respectively, in which, 24/31 of Rb protein positive tumors showed null or low expression of p16 protein,while 19/23 of Rb negative tumors were associated with positive or high level expression of p16 protein. Conclusions (a). p16 and Rb proteins were both involved in astrocytoma progression. (b) Negatively correlated pressionof p16 and Rb protein might be one of characteristics of malignant astrocytoma.

关 键 词：星形细胞瘤 P16 RB蛋白 视网膜母细胞瘤 表达 

分 类 号：R739.720.2[医药卫生—肿瘤]