p53蛋白在宫颈鳞状上皮内病变与宫颈癌中的过度表达及其与HPV的关系  

Overexpression of p53 protein and its relation to HPV in squamous intraepithelial lesion and cervical carcinoma

作　　者：李新英[1,2,3] 糜若然[1,2,3] 焦书竹[1,2,3] 贾宗智 

机构地区：[1]天津医科大学总医院妇产科 [2]北京天坛医院妇产科 [3]山西长治医学院病理学教研室

出　　处：《中华病理学杂志》1997年第6期360-363,共4页Chinese Journal of Pathology

摘　　要：目的探讨宫颈鳞状上皮内病变（ＳＩＬ）和宫颈癌中抑癌基因ｐ５３蛋白表达水平以及与ＨＰＶ的关系。方法用免疫组化和ＰＣＲ－ＲＦＬＰ方法，对２６８例宫颈石蜡包埋组织（２９例慢性宫颈炎、６８例ＳＩＬ、１７１例宫颈癌）进行了ｐ５３蛋白水平及多型ＨＰＶ检测。结果宫颈癌ｐ５３蛋白的过度表达率及强阳性过度表达率高于ＬＳＩＬ和ＨＳＩＬ，ＨＰＶ１８阳性的ＳＩＬ及宫颈癌ｐ５３蛋白的过度表达率高于ＨＰＶ１６阳性和ＨＰＶ阴性者。结论抑癌基因ｐ５３蛋白过度表达率与宫颈癌的发生发展有关，与ＨＰＶ１８的内在联系尚有待于进一步研究。Objective To study the relation between the expression of p53 protein and HPV infection in squamous intraepithelial lesion (SIL) and cervical carcinoma. Methods The expression of p53 protein was determined in the paraffin embedded tissues of 171 cases of cervical carcinoma, 68 cases of SIL and 29 cases of chronic cervicitis, using ABC immunohistochemical method. Detection and typing of HPV were carried out adopting PCRRFLP technique on the same tissues.Results In the cervical carcinomas, the overexpression rate (38.6%) and intensive overexpression rate (11.7%) of p53 protein were higher than those in LSIL (10.0% and 0.0%, respectively) and HSIL (35.4% and 2.1%, respectivly). The overexpression rates of p53 protein in SIL and cervical carcinoma with positive HPV 18 were 87.5% and 84.3% respectively, higher than 21.2% and 10.5% in the corresponding groups carrying HPV 16, as well as 16.7% and 57.1% in the corresponding groups negative for HPV. Conclusions The overexpression of p53 protein might play a role in the carcinogenesis of cervical carcinoma. The relation between p53 overexpression and HPV 18 infection in SIL and cervical carcinoma remains to be clarified.

关 键 词：宫颈肿瘤 P53蛋白 乳头状瘤病毒 表达 

分 类 号：R737.330.2[医药卫生—肿瘤]