低剂量辐射诱导EL-4淋巴瘤细胞凋亡及细胞周期进程适应性反应的剂量率效应  

Dose-rate effect of adaptive response of apoptosis and cell cycle progression induced by low-dose ionizing radiation in EL-4 lymphoma cells in vitro

作　　者：刘淑春[1] 赵文举[2] 吕喆[1] 李艳博[1] 康顺爱[1] 龚守良[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021 [2]解放军第208医院妇产科,吉林长春130062

出　　处：《吉林大学学报（医学版）》2008年第1期24-27,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30570546)

摘　　要：目的:观察低剂量辐射诱导EL-4淋巴瘤细胞凋亡及细胞周期进程适应性反应的剂量率效应,以揭示低剂量辐射生物效应及其诱导适应性反应的可能机制。方法:实验分D2组(攻击剂量)、D1(诱导剂量)+D2组和假照组。用X射线照射离体EL-4淋巴瘤细胞,其D1为75mGy(剂量率6.25～200.00mGy·min-1),D2为1.5Gy(剂量率287mGy/min),D1和D2间隔6h。通过流式细胞仪检测其细胞凋亡和细胞周期进程的变化。结果:当D1剂量率为6.25～50.00mGy,D1+D2组细胞凋亡百分数明显低于D2组(P<0.05),G0/G1期细胞百分数明显低于D2组(P<0.01),而S期细胞百分数不同程度高于D2组。结论:D1为75mGy(剂量率6.25～50.00mGy·min-1),D2为1.5Gy(剂量率287mGy·min-1)、D1和D2间隔6h,可诱导体外EL-4淋巴瘤细胞凋亡和细胞周期进程的适应性反应。Objective To observe the dose-rate effect of adaptive response of apoptosis and cell cycle progression induced by low-dose ionizing radiation in EL-4 lymphoma cells in vitro in order to reveal the possible mechanism of biological effect and adaptive response induced by low dose radiation. Methods The experiment was divided into D2 （challenging dose）, D1 （inductive dose） ＋ D2 and sham-irradiation groups. EL-4 lymphoma cells were irradiated with D1 （75 mGy, 6.25--200.00 mGy · min 1） and D2 （1.5 Gy, 287 mGy - min 1）, the time interval between D1 and D2 was 6 h. The percentage of apoptosis and each cell cycle phase were measured with flow cytometry. Results When the dose rates of D1 were 6.25--50.00 mGy · min-1 , the percentages of apoptosis in the D1 ＋ D2 group were significantly lower than those in the D2 group （P〈0.05） ; meantime, the percentages of G0/G1 phase cells decreased significantly （P〈 0.01）, and the percentages of S phase cells increased at different degrees. Conclusion When the dose rates of D1 （75 mGy） are 6. 25--50. 00 mGy.min^-1, D2isl. SGy（287mGy.min^-1）, and the time interval between 131 and D2 is 6 h, the adaptive response of apoptosis and cell cycle progression in EL-4 lymphoma cells in vitro could be induced.

关 键 词：电离辐射 淋巴瘤细胞 剂量率 细胞凋亡 细胞周期 适应性反应 

分 类 号：R733.4[医药卫生—肿瘤]