B段紫外线对豚鼠皮肤的损伤作用  

Effects of ultraviolet irradiation on skin of guinea pig

作　　者：张雯雯[1] 刘雅娟[2] 李云[3] 陈强[1] 李鹏[1] 凌翎[1] 林晓晨[4] 任淑萍[1] 

机构地区：[1]吉林大学公共卫生学院毒理学教研室,吉林长春130021 [2]吉林大学公共卫生学院营养与食品卫生学教研室,吉林长春130021 [3]吉林大学中日联谊医院眼科,吉林长春130033 [4]吉林大学畜牧兽医学院

出　　处：《吉林大学学报（医学版）》2008年第1期46-48,F0002,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30070705);吉林省卫生厅基金资助课题(2006003)

摘　　要：目的:探讨B段紫外线(UV-B)对豚鼠皮肤的损伤作用。方法:实验动物随机分为UV-B照射组和未照射对照组,通过大体观察、组织切片及皮下组织中氧化损伤指标MDA、超氧阴离子和羟自由基的测定,探讨UV-B对豚鼠皮肤的损伤作用。结果:照射组豚鼠皮肤表皮红肿、脱屑;照射剂量达5.28J/cm2时,组织切片中可见整个表皮层增厚,内含排列紊乱的不典型细胞,层次不清,表面有角化过度和角化不全;棘细胞层明显肥厚。全层细胞体积均增大且具异型性,核大小不一,形态各异,染色深浅不匀。UV-B照射组血清和皮下组织中MDA和ROS含量均显著高于对照组(P<0.05)。结论:UV-B能够造成豚鼠皮肤的光老化并出现癌前病变。Objective To explore the adverse e{{ects o{ ultraviolet B （UV-B） on the skin of guinea pig. Methods Guinea pig skin was irradiated with UVB, the skin changes in external appearance, pathology, and the contents of OH and O2- produced in the skin were determined to study the adverse effects of UV-B on the guinea pig skin. Results UV B caused red swelling and desquamation of skin, with the increasing of the UV-B irradiation, the cells in stratum spinosum began to proliferate vigorously, the MDA and ROS contents in UVB radiation group were significantly higher than those in control group （P〈0.05）. Conclusion UV-B can cause injury to guinea pig skin and has the potential to produce skin cancer.

关 键 词：紫外线 豚鼠 自由基 氧化损伤 

分 类 号：R363.122[医药卫生—病理学]