石榴皮提取物对小鼠氧化应激状态的改善作用  

Effect of Pomegranate Peel Extracts on Oxidative Stress in Restrained Mice

作　　者：徐真真[1] 冯锦和 陆惠文 宝丽[3] 栗原博[1] 

机构地区：[1]暨南大学中药及天然药物研究所 [2]广东罗浮药谷有限公司研究所,广东惠州516127 [3]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《中药材》2008年第8期1193-1196,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:研究石榴皮提取物(pomegranate peel extracts,PPE)对拘束负荷引起小鼠氧化应激性状态的改善作用。方法:18 h拘束负荷诱发小鼠应激性肝损伤,通过测定小鼠血浆中丙氨酸氨基转移酶、谷胱甘肽、丙二醛水平及肝组织的抗氧化能力指数,观察石榴皮提取物对小鼠氧化应激性状态的改善作用。结果:与拘束模型组相比,石榴皮提取物能有效降低小鼠血浆中丙氨酸氨基转移酶和丙二醛水平,明显提高拘束负荷小鼠肝组织的抗氧化能力指数和血浆谷胱甘肽水平。结论:石榴皮提取物对拘束负荷引起的小鼠应激性肝损伤具有一定的改善作用,其作用机制可能与石榴皮提取物清除自由基、抑制脂质过氧化及缓解体内氧化应激状态有关。Objective ： To observe the protective effects of pomegranate peel extracts on oxidative stress in restrained mice. Methods： The liver injury model was established under 18 h restraint stress in mice. The protective effects were evaluated by assessing alanine aminotransferase （ALT） GSH and Malondialdehyde （MDA） levels in plasma and antioxidant capacities of liver were measured by using oxygen radical absorbance capacity （ORAC） assay. Results ： Activities of ALT and levels of MDA were observed to decrease on pomegranate peel extracts treated mice as compared with restraint stress control group and antioxidant capacity in liver homogenization and the levels of GSH in plasma significantly increased as compared with restraint stress. Conclusion： The results indicate that pomegranate peel extracts exert protective effects on oxidative stress in mice loaded with restraint stress, which may be related to its free radical scavenging activity and lipid peroxidation inhibitory effect.

关 键 词：石榴皮提取物 拘束应激 肝损伤 抗氧化能力指数 

分 类 号：R285.5[医药卫生—中药学]