全反式维甲酸诱导胶质瘤细胞增殖相关基因的差异表达  

Differentially expressed genes related to proliferation in glioma cells induced with all-trans retinoic acid

作　　者：彭涛[1] 曾义[1] 徐宏[1] 周增俊[1] 游潮[2] 

机构地区：[1]德阳市人民医院神经外科,四川德阳618000 [2]四川大学华西医院神经外科,四川成都610041

出　　处：《西部医学》2008年第5期911-912,共2页Medical Journal of West China

基　　金：四川省卫生厅科研项目(No.050209)

摘　　要：目的筛选全反式维甲酸诱导胶质瘤细胞所致增殖相关差异表达基因,为进一步研究胶质瘤进展的基因通路及机制提供依据。方法通过运用cDNA微阵列技术比较10μmol/L全反式维甲酸处理前后胶质瘤SHG-44细胞(WHO级)的基因表达谱,鉴定与胶质瘤增殖相关的差异表达基因。随机选择数个差异表达基因进行Northern杂交实验,以验证cDNA微阵列结果的可靠性。结果实验鉴定出42个差异表达基因,其中表达上调28个、下调14个。5个差异表达基因:MDM2、PDCD5、SOD2、SERPINF1和MAPK11与胶质瘤的增殖相关。结论初步揭示全反式维甲酸能诱导胶质瘤细胞增殖相关基因的差异表达,提示前述差异表达基因可能与胶质瘤的进展有关。Objective To provide basic data for further research on genetic pathways and genetic mechanism of glioma progression with screening the differentially expressed genes related to proliferation in glioma cells induced with all-trans retinoic acid （ATRA）. Methods To comparing the gene expression profiling of glioma SHG-44 cells （WHO grade Ⅳ ） before and after treatment with ATRA with cDNA microarray, a panel of differentially expressed genes that related to glioma proliferation was identified. To confirm the results of genes detected with cDNA microarray, some differentially expressed genes were randomly selected for Northern blot. Results Forty-two differential genes were identified with cDNA microarray in the present study, Among them, there were 28 were up-regulated and 14 downregulated. The five differentially expressed genes, including MDM2, PDCD5, SOD2, SERPINF1, and MAPK11, involved in glioma proliferation. Conclusion It is primarily revealed that ATRA might induce differentially expression of prollferation-related genes in glioma cells, which suggests that the above-mentioned differentially expressed genes might correlate with glioma progression.

关 键 词：基因 SHG-44 胶质瘤 全反式维甲酸 CDNA微阵列 

分 类 号：R739.41[医药卫生—肿瘤]