苦参碱对人涎腺腺样囊性癌ACC-M细胞增殖抑制的研究  

Study on inhibitory effect of matrine to human salivary adenoid cystic carcinoma ACC-M cells

作　　者：赵军方[1] 李峰[1] 李新明[1] 王海斌[1] 方政[1] 李青川[1] 

机构地区：[1]郑州大学第一附属医院口腔颌面外科,河南郑州450052

出　　处：《国际口腔医学杂志》2008年第4期361-362,368,共3页International Journal of Stomatology

基　　金：河南省教育厅自然科学基金资助项目(2008A320035)

摘　　要：目的探讨中药有效成分苦参碱(Mat)对人涎腺腺样囊性癌(SACC)细胞系ACC-M细胞增殖的抑制作用。方法体外培养ACC-M细胞,用不同质量浓度Mat溶液对体外培养的ACC-M细胞进行处理。通过MTT比色法和流式细胞仪细胞周期检测来测定不同质量浓度Mat溶液对ACC-M细胞增殖的抑制作用。结果经Mat处理后,ACC-M细胞生长明显受到抑制,细胞周期阻滞于G0/G1。结论Mat对ACC-M细胞的增殖有显著的抑制作用,其抑制作用的发生可能与其细胞周期阻滞有关。Objective To investigate the effects of matrine on inhibition of cell proliferating in human adenoid cystic carcinoma cell line ACC-M. Methods ACC-M was cultured in vitro by different concentration matrine. Inhibitory effect of proliferation was investigate in light microscope, by the way of MTT and flow cytometry analysis. Results Proliferation of ACC-M cells was inhibited obviously and cell cycle was arrested by treatment of matrine. Conclusion Matrine could obviously inhibit the proliferation of ACC-M cells. One of mechanisms could be cell cycle arrested for inhibition of cell proliferation.

关 键 词：苦参碱 腺样囊性癌 增殖抑制 细胞周期 

分 类 号：R739.8[医药卫生—肿瘤]