五氯酚和八氯代二苯并二噁英复合暴露对斑马鱼胚胎发育的毒性效应  

Combined Toxicity of Pentachlorophenol(PCP)and Octachlorodibenzo-p-dioxin(OCDD)on Development of Zebrafish(Danio rerio)Embryos

作　　者：蒋琳[1] 周珍[1] 胡俊[2] 吴艳娣[3] 张捷[3] 梁勇[1] 郑明辉[4] 

机构地区：[1]江汉大学医学院,武汉430056 [2]南京大学环境学院,南京210093 [3]华中农业大学资源与环境学院,武汉430070 [4]中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室,北京100085

出　　处：《生态毒理学报》2008年第4期370-376,共7页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金项目(No.40332023);武汉市青年科技晨光计划项目(No.20055003059-11)

摘　　要：为了评价环境中五氯酚(PCP)和八氯代二苯并二噁英(OCDD)对水环境以及鱼类的影响,以斑马鱼为模式生物,研究了PCP和OCDD对其胚胎发育的单一及复合毒性效应.结果表明,PCP单独暴露(浓度25μg·L-1～5mg·L-1)对斑马鱼胚胎发育具有较强的毒性效应,可导致胚胎孵化率显著下降,死亡率、畸形率显著上升,而OCDD单独暴露(200、500μg·L-1)对斑马鱼胚胎发育没有明显的毒性效应;OCDD与环境浓度的PCP复合暴露(OCDD+PCP1:250μg·L-1+25μg·L-1;OCDD+PCP2:250μg·L-1+50μg·L-1)对斑马鱼胚胎的存活与发育等没有显著影响,对斑马鱼胚胎内CYP1A基因表达以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的酶活力也没有显著影响,在实验浓度下二者共存没有明显的复合毒性效应。In order to assess the effect of pentachlorophenol （PCP） and octachlorodibenzo-p-dioxin （ OCDD ） on aquatic environment and fish, we studied the single and combined toxicity of PCP and OCDD using zebrafish as the model animal. The results showed that PCP（25μg·L^-1-5mg·L^-1）could cause severe developmental toxicity, while no effects were observed by OCDD（200μg·L^-1 and 500μg·L^-1）. Embryos exposed to PCP showed a significant reduction in hatching rate while increase in mortality and malformation rate. No obvious effect on survival and development was found for the combined exposure of PCP and OCDD（OCDD＋PCP1： 250μg·L^-1＋25μg·L^-1; OCDD＋PCP2： 250μg·L^-1＋50μg·L^-1）, and there were no significant differences in CYP1A expression and activities of SOD and CAT between the co-exposed embryos and the control. In conclusion, our results showed that there was no obvious combined toxicity to zebrafish embryos at the experimental exposure concentrations.

关 键 词：PCP OCDD 复合毒性 斑马鱼 胚胎 

分 类 号：X171[环境科学与工程—环境科学]