柚皮素对K562细胞的抑制作用与Caspase酶活性、PCNA蛋白表达的关系  

Relation of Apoptosis of K562 Induced by Naringenin to Expression of PCNA and Enzyme Activity Changes of Caspase

作　　者：刘明辉[1] 左学兰[1] 胡宁[1] 周颖[1] 冯颖倩[1] 

机构地区：[1]武汉大学中南医院血液内科,湖北武汉430071

出　　处：《医学新知》2008年第4期201-202,205,共3页New Medicine

摘　　要：目的本研究旨在探讨柚皮素对K562细胞的生长抑制作用与Caspase酶的活性及细胞增殖核抗原(PCNA)蛋白表达的关系。方法用50mol/L,200mol/L的柚皮素分别作用K562细胞后,Caspase分光光度计法检测细胞内Caspase-3和Csapase-9酶活性的改变;用细胞免疫化学的方法检测PCNA蛋白的表达。结果在柚皮素作用下,K562细胞内Caspase-3和Caspase-9活性增强(P<0.05),PCNA的表达显著降低(P<0.05)。结论柚皮素对K562细胞的抑制作用可能是通过细胞增值抑制和上调Caspase-9和Csapase-3活性诱导细胞凋亡而实现。Objective To investigate the relation ofapoptosis ofK562 cells induced by naringenin to expression of PCNA and enzyme activity of Caspase. Methods The activity of Caspase-3 and Caspase-9 in K562 cell line induced by naringenin was detected by Caspase Colorimetric Assay. PCNA protein ofK562 cell line treat- ed by naringenin was detected by immunhistochemical staining. Results The activity of Caspase-3 and Caspase-9 increased remarkably with treatment ofnaringenin, while PCNA protein in treatment group decreased remarkably than that in control group. Conclusion The activity of Caspase-3 and Caspase-9 and cell cycle arrest may contribute to the apoptosis of K562 cell in vitro induced by naringenin.

关 键 词：柚皮素 CASPASE-3 CASPASE-9 PCNA 

分 类 号：R733.7[医药卫生—肿瘤]