剑叶龙血素B对辣椒素诱发大鼠背根神经节细胞VR1电流的抑制作用  

Inhibition of Cochinchinenin B on Capsaicin-Activated VR1 Currents in Dorsal Root Ganglion Neurons of Rats

作　　者：陈素[1] 王松涛[1] 刘向明[1] 

机构地区：[1]中南民族大学生物医学工程系,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2008年第2期52-55,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家民委自然科学基金资助项目(MZY06002)

摘　　要：使用全细胞膜片钳技术,在急性分离的大鼠背根神经节细胞上,观察了剑叶龙血素B(CB)对辣椒素(CAP)诱发电流和电压反应的影响.在-60 mV的钳制电压下,剑叶龙血素B快速、可逆地抑制了辣椒素诱发电流,并且该抑制呈浓度依赖性,半数抑制浓度(IC50)为0.92 mmol/L;在电流钳条件下,剑叶龙血素B抑制了辣椒素诱发的去极化.0.38 mmol/L的剑叶龙血素B产生的抑制率为38.6±1.9%,而0.76 mmol/L的剑叶龙血素B几乎全部抑制了辣椒素诱导的去极化.这些结果表明:剑叶龙血素B对CAP/VR1反应的抑制可能用来解释一些血竭的镇痛效应.The modulations of cochinchinenin B on capsaicin-activated currents （ICAP） and voltage responses were investigated in acutely isolated dorsal root ganglion （DRG） neurons of rats by using whole-cell patch clamp technique. Under voltage-clamp condition, eochinchinenin B rapidly and reversibly inhibited the ICAP, in a concentration-dependent manner with an IC50 of 0.92 mmol/L. Under current-clamp condition, cochinchinenin B inhibited capsaicin-activated depolarizations. The capsaicin-evoked depolarizations were reduced 38.6± 1.9% with 0.38 mmol/L cochinchinenin B. The depolarizations were completely blocked when 0.76 mmol/L cochinchinenin B was applied. These results suggested that the inhibition on the capsaicin-induced responses of cochinchinenin B could be useful to explain some of the analgesic effects of dragon＇s blood resin.

关 键 词：剑叶龙血素B 辣椒素 全细胞膜片钳技术 背根神经节 

分 类 号：R971.1[医药卫生—药品]