人口腔鳞癌手术标本组织块裸鼠移植瘤模型的建立  

Establishment of a Transplanted Tumor Model of Human Oral Squamous Cell Carcinoma in Nude Mice

作　　者：张燕[1] 张萍[1] 陈万涛[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院.上海交通大学口腔医学院.上海市口腔医学研究所口腔颌面外科,上海200011

出　　处：《口腔颌面外科杂志》2008年第4期256-260,共5页Journal of Oral and Maxillofacial Surgery

基　　金：上海市重点学科(优势学科)建设项目资助(Y0203);国家自然科学基金重点项目(30330580);国家自然科学基金面上项目(30772433);上海市科委科研计划项目(07QA14038)

摘　　要：目的:口腔鳞癌是口腔颌面部常见的恶性肿瘤之一,建立与人类口腔癌相似的动物模型,对进一步探讨口腔癌的发生、发展、治疗和预后具有重要意义。方法:选取新鲜的口腔鳞癌手术标本,制备成1mm^3组织块,植入BALB/c nu-nu裸鼠皮下,观察其成瘤情况,并对移植瘤的生长特性、组织形态和对化疗药物的反应进行检测。结果:收集了27例临床标本,成功建立了15个裸鼠皮下移植瘤模型,成瘤率为56%。所建立的口腔鳞癌移植瘤模型能够在裸鼠体内连续成瘤。与细胞系建立的移植瘤模型相比,其组织学特征与人口腔鳞癌标本极为相似。其中,20%(2/10)的移植瘤模型对传统化疗药物顺铂敏感。结论:成功建立了人口腔鳞癌手术标本组织块移植瘤模型。该模型为进一步研究口腔鳞癌提供了基础。Objective： To establish a transplanted tumor model of human oral squamous cell carcinoma in nude mice. Methods： 27 fresh histologically intact tumor tissues were obtained during operations. Tissues were cut into small pieces about 1 mm3.The xenografts were subcutaneously transplanted into BALB/c nu-nu nude mice and observed periodically. Their sensitivity to common chemotherapeutic drugs were tested. Results： In 27 xenografts transplanattion,15 tumor models in nude mice were successfully established. The tumor formation rate is 56%, The histological characteristics between human tumor and animal model tumor were similar. 20% transplanted tumors were sensitive to traditional chemotherapeutic drug cisplatin. Conclusion： We successfully established a human oral squamous cell carcinoma transplanted tumor model with operation tumor samples.

关 键 词：鳞状细胞癌 顺铂 动物模型 

分 类 号：R739.8[医药卫生—肿瘤]