广西莪术挥发油化学成分的分析  

Analysis on Chemical Constituents of Essential Oil from Curcuma Kwangsiensis

作　　者：陈旭[1] 曾建红[1] 戴平[1] 葛静[2] 

机构地区：[1]桂林医学院药学院,广西桂林541000 [2]广西中医学院药学院,广西南宁530001

出　　处：《药物生物技术》2008年第4期293-295,共3页Pharmaceutical Biotechnology

基　　金："广西莪术质量与道地产区生态环境的研究"广西自然科学基金(桂科字0447101)

摘　　要：采用气相色谱-质谱联用仪(GC-MS)对广西莪术挥发油进行分离测定。色谱柱HP-5MS毛细管柱(30.0m×250μm×0.25μm),进样温度250℃,不分流;程序升温:初始65℃恒温2min,以5℃/min升至90℃恒温3min,以20℃/min升至103℃恒温3min,以8℃/min升至150℃恒温15min,以20℃/min升至280℃。结合计算机检索对分离的化合物进行结构鉴定,用色谱峰面积归一化法测定各成分的相对百分含量。结果共分离得到52个气相色谱峰,鉴定出20种化学成分,其中β-榄香烯、莪术酮、莪术醇、莪术二酮4种主要抗肿瘤活性成分均能检测到。此方法可用于莪术原料药材和莪术挥发油制剂的检测和相对含量测定。To study chemical constituents of essential oil and to determine main components of antitumor activity from Curcuma kwangsiensis, the chemical constituents of essential oil were separated and identified by GC-MS on a HP-5 column（30. 0 m × 250 μm × 0. 25 μm）with, Inlet temperature 250℃, splitless. Temperature programming started at 65℃, holding for 2 rain, then increased at 90℃ at a rate of 5℃/min, and then increased at 103℃ at a rate of 20℃/min, holding for 3 rain, after that increased at 150℃ at a rate of 8℃/min, holding for 15 min, and finally increased at 280℃ at a rate of 20℃/min. The relative contents in the essential oil were identified by area nomalizaion. Fifty-two peaks appeared in GC spectra. Twenty chemical constituents were determined and four components of anti-tumor activity were determined： β-elemene, curdione and curcumol, curzerenone. The method can be used in detecting and assaying the content for thedrug of curcuma materials and its preparation.

关 键 词：广西莪术 挥发油 气相色谱-质谱 抗肿瘤活性成分 

分 类 号：Q78[生物学—分子生物学]