蛴螬提取物对人肺癌A_(549)细胞诱导凋亡作用的形态学观察  

Morphological observation of A_(549) human lung cancer cells by the Raw Grub extract

作　　者：崔春爱[1] 孙抒[1] 杨万山[1] 

机构地区：[1]延边大学基础医学院,吉林延吉133000

出　　处：《山东医药》2008年第27期7-8,共2页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30460047);教育部科学技术研究重点资助项目(03033)

摘　　要：目的探讨蛴螬提取物对人肺癌A549细胞诱导凋亡的形态学改变。方法应用MTT法、HE染色、扫描电镜的方法观察蛴螬提取物对体外培养的人肺癌A549细胞形态学影响。结果蛴螬石油醚提取物对人肺癌A549细胞株的增殖抑制作用呈剂量依赖性和时间依赖性(P<0.05或P<0.01)。倒置显微镜下蛴螬石油醚提取物80μg/ml作用后,早期细胞出现凋亡形态学变化,晚期细胞发生膜破裂坏死。HE染色和扫描电镜观察细胞出现典型凋亡形态变化。结论蛴螬提取物在体外对人A549肺癌细胞株有显著性抑制增殖和诱导凋亡作用。Objective To observe the morphological changes of the apoptosis and degeneration of A549 human lung cancer cells in vitro after treatment with the raw extract of Grub. Methods The growth inhibition rates and cytotoxic effects of A549 cells were measured with MTT assay. Morphology changes were observed by microscope, HE and scan electron microscope. Results The Raw Grub extract could significantly inhibit the growth and proliferation of A549 cells in dose-dependent and time-dependent manner. Inverted microscope revealed that the A549 cells showed the apoptosis changes after treatedment with 80μg/ml of the Grub petroleum ether extract before 24 h and necrosised; after 72 h. Conclusion The Grub extract has an influence on apoptosis induction and prdiferation inhibition of A549 human lung cancer cells in vitro.

关 键 词：蛴螬提取物 A549 肺癌细胞株 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]