大黄素对卵巢癌HO-8910PM细胞中PRL-3和NAG-1表达的影响  

Effect of emodin on expression of PRL-3 and NAG-1 in HO-8910PM cells

作　　者：何太平[1] 莫丽儿[2] 梁念慈[1] 

机构地区：[1]广东医学院生物化学与分子生物学研究所,广东湛江524023 [2]广东天然药物研究与开发重点实验室

出　　处：《山东医药》2008年第27期12-14,共3页Shandong Medical Journal

基　　金：广东省科技计划项目(2007B030702003);广东省中医药局课题(1060044);广东省卫生厅课题(B2005104)

摘　　要：目的研究大黄素对人高转移卵巢癌HO-8910PM细胞中促肝细胞再生磷酸酶-3(PRL-3)和非类固醇抗炎药物激活基因(NAG-1)表达的影响。方法采用Western blot法检测大黄素对HO-8910PM细胞中PRL-3和NAG-1蛋白表达的影响,荧光定量实时PCR分析大黄素对HO-8910PM细胞中PRL-3和NAG-1 mRNA表达的影响。结果大黄素能明显下调HO-8910PM细胞中PRL-3的表达,强烈上调NAG-1表达。结论大黄素影响高转移卵巢癌细胞转移能力可能与PRL-3和NAG-1表达有关。Objective To investigate the effect of emodin on expression of PRL-3 and NAG-1 in human highly metastatic ovarian carcinoma HO-8910PM ceils in vitro. Methods The expression level of PRL-3 and NAG-1 proteins were assessed by Western blot analysis. Huorescent quantitative real-time PCR assay was performed to determine the effect on expression of PRL-3 and NAG-1 mRNA by emodin. Results Emodin distinctly down-regulated the expression of PRL-3, significiantly up-regulated NAG-1 mRNA expression. Conclusion The possible mechanism for inhibitory effect of emodin on metastasis in vitro is involved in PRL-3 and NAG-1 expression.

关 键 词：大黄素 卵巢肿瘤 聚合酶链反应 

分 类 号：R737.31[医药卫生—肿瘤]