荧光原位杂交同时检测精子X和Y染色体的方法  

Simultaneous detection of X-and Y-bearing human sperm by double fluorescence in situ hybridization

作　　者：李善国[1] 施祖美[1] 邵敬於[1] 左伋[2] 曹凤根[2] 

机构地区：[1]上海市第一妇婴保健院,200040 [2]上海医科大学医学遗传中心,200032

出　　处：《中国优生与遗传杂志》1997年第5期4-6,共3页Chinese Journal of Birth Health & Heredity

摘　　要：用实验手段分离X和Y染色体精子或富集特定性别染色体精子．对于预防性连锁遗传病具有潜在应用价值。建立精子X和Y染色体鉴定方法对于评估分离或富集特定性别染色体效果至关重要。本文介绍采用CY3TM和FlourXTM探针作荧光原位杂交（FISH），同时检测精子Y和X染色体。10份正常精液标本分析结果显示：X染色体精子为50．23％，Y染色体精子为49．77％；有效杂交率达91．99％。FISH方法比传统的精子染色体核型分析和奎纳克林染色检查Y荧光小体更具有简易、特异和快速的优点。Separation of X-bearing human sperm from Y-bearing sperm or enrichment of special sex sperm for the gender preselection is potentially valuable for the prevention of X-linked diseases. It is very important to assess the reliability of separation or enrichment by identifying the X or Y chromosome sperm. In the study,f Iurescence in situ hybridization (FISH ) with Fluro XTM X chromosome and Cy3TM Y chromosome probe was intreduced. The result from 10 normal semen samples show that X-bearing sperm is 50. 23% and Y-bearing sperm is 49. 77 %,the hybridization rate reaches 91. 99% on the average. FISH is simpler,more reapid and speific in detection of sperm sex chromosome than traditional karyotyping and Y they with quinacrine staining.

关 键 词：遗传性疾病 荧光原位杂交 精子 X染色体 Y染色体 

分 类 号：R446.19[医药卫生—诊断学] R698[医药卫生—临床医学]