应用英国药典2005版中薄层色谱法鉴别注射用头孢呋辛钠  

Identification of Cefuroxime Sodium for Injection by TLC Method According to BP 2005

作　　者：邹文博[1] 许明哲[1] 李娅萍[1] 胡昌勤[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《中国药品标准》2008年第4期293-294,共2页Drug Standards of China

摘　　要：目的:应用英国药典2005版中注射用头孢呋辛钠鉴别项下薄层色谱法鉴别注射用头孢呋辛钠。方法:使用GF254硅胶薄层板,展开剂为15%pH6.2醋酸铵溶液-四氢呋喃,试验了9∶1,5∶5,3∶7三种比例,点样量1微升,于254nm紫外灯下检视。结果:以15%pH6.2醋酸铵溶液-四氢呋喃(3∶7)为展开剂可以使系统适用性溶液中头孢呋辛和头孢西丁主斑点完全分离,达到鉴别目的。结论:经调整展开剂比例后,该薄层色谱系统可以有效鉴别注射用头孢呋辛钠。Objectives： To identify Cefuroxime Sodium for Injection by TLC method according to BP 2005. Methods： Using GF254 as coating substances and as the mobile phase a mixture of 15.0% w/v solution of ammonium acetate, previously adjusted to pH6. 2 with glacial acetic acid, and tetrahydrofuran with the proportion of 9： 1,5：5 and 3： 7. Apply to the plate 1 μL of the solutions and examine under ultraviolet light（254 nm） after removal of the plate. Results： The result of system suitability test turned out to be two separated principal spots which are cefuroxime and eefxitin using a mixture of 3 volumes of 15. 0% w/v solution of ammonium acetate, previously adjusted to pH6. 2 with glacial acetic acid, and 7 volumes of tetrahydrofuran as the mobile phase. Conclusions： The TLC identification system can identify Cefuroxime Sodium for Injection effectively by adjusting the proportion of aqueous phase and organic phase of the mobile phase.

关 键 词：头孢呋辛钠 薄层鉴别 薄层色谱法 硅胶薄层板 

分 类 号：R978.11[医药卫生—药品] R286.0[医药卫生—药学]