优福定节拍性化疗抗MKN-45荷瘤裸鼠血管生成作用的研究  

Antiangiogenic effect of metronomic UFT in human MKN-45 gastric cancer cell line

作　　者：吴欣[1] 王喜[1] 于观贞[1] 陈颖[2] 姚明[3] 闫明霞[3] 王杰军[1] 

机构地区：[1]第二军医大学附属长征医院肿瘤科,上海200070 [2]上海第二军医大学附属长海医院病理科,200070 [3]上海市肿瘤研究所实验病理研究室,200433

出　　处：《临床肿瘤学杂志》2008年第8期682-685,共4页Chinese Clinical Oncology

基　　金："十一五"国家科技支撑计划重大项目(2006BA102A05)

摘　　要：目的:研究持续小剂量优福定(UFT)对MKN-45荷瘤裸鼠的疗效及其机制。方法:建立荷瘤动物模型,用UFT和环磷酰胺(CTX)对荷瘤动物模型进行治疗,观察其疗效及毒副作用;流式细胞术检测外周血内皮祖细胞(CEP);免疫组化检测微血管密度(MVD)。结果:持续小剂量UFT明显抑制肿瘤的生长,CEP和MVD含量下降显著,而持续小剂量CTX虽然抑瘤率较持续小剂量UFT高,但却未抑制CEP和MVD含量。结论:持续小剂量UFT可通过抑制血管生成对MKN-45胃癌细胞荷瘤裸鼠产生抗瘤效应。Objective：To assess effect of metronomic UFT in human MKN-45 gastric cancer cell line. Methods：After the establishment of animal models, CEPs were measured by flow cytometry. After mice were treated with UFT and CTX,mice were monitored regularly, and were killed, evaluation of CEPs was carried out on blood by flow cytometry, tumor angiogenesis was evaluated in parallel by immunohistochemistry. Results：We found that metronomic UFT inhibit the mobilization of CEPs and MVD,but metronomic CTX didn＇t inhibit it, although in group CTX LMD, inhibiton rate of tumor was higher than in group UFT LMD. Conclusion：Metronomic UFT has antiangiogenic effect in human MKN-45 gastric cancer cell line.

关 键 词：裸鼠 节拍性化疗 内皮祖细胞 微血管密度 

分 类 号：R735.7[医药卫生—肿瘤]