应用SELDI-TOF-MS技术筛选肾细胞癌患者血清标志物  

Screening on the tumor related markers in the serum of the renal cell carcinoma patients by the SELDI-TOF-MS technique

作　　者：郝强[1] 赵继懋[1] 杜林栋[1] 

机构地区：[1]首都医科大学附属北京友谊医院泌尿外科

出　　处：《中国癌症杂志》2008年第8期596-599,共4页China Oncology

摘　　要：背景与目的:表面增强激光解吸离子化飞行时间质谱(SELDI-TOF-MS)技术有助于筛选肾细胞癌相关的分子变化。本研究探讨人肾细胞癌患者血清和正常人血清之间蛋白质表达的差异,筛选特异性生物标志物。方法:采用SELDI-TOF-MS技术,选用WCX2蛋白质芯片对28例肾透明细胞癌及28例正常对照组血清标本进行检测以筛选肾癌患者血清中差异表达蛋白质。结果:在分子量1.5×103～30×103(1.5～30kD)范围内,检测出170个差异蛋白峰。肾透明细胞癌差异表达明显的蛋白峰7个(P<0.01),其中低表达的3个,相对分子量分别为4.098×103、5.917×103、6.643×103,高表达的4个,相对分子质量为5.572×103、6.344×103、6.529×103、8.518×103。结论:人肾肿瘤血清中特异性蛋白质的发现,对肿瘤临床特异性生物标志物的检测及进一步研究肿瘤发生机制等具有重要意义。Background and purpose： Surface enhanced laser desorption ionization time of flight mass spcctrometry （SELDI-TOF-MS） is useful in helping to identify the molecular changes closely related to renal cell carcinoma. We explored the different expression of sera protein between human renal cell carcinoma patients and normal to screen renal cancer-specific biomarkers. Methods： The protein mass spectrometry of 28 cases with renal cell carcinoma and 28 normal persons were detected by WCX2 protein chip combining with SELDI-TOF-MS technique for screening the different proteins. Serum samples from 28 patients with clear renal cell carcinoma and 28 normal persons were used to detect biomarkcrs for clear renal cell carcinoma by SELDI-TOF-MS technique with WCX2 Protcinchip. Results： 170 effective protcin wavc crests between 1.5×10^3-30×10^3（1.5-30 kD） were detected. Seven proteins were specifically detectcd in sera of patients with clear renal cell carcinoma, but not in normal donor. The protcins with MW 4.098,5.917,6.643 ×10^3 were down-regulated ,and four proteins with MW 5.572,6.344,6.529,8.518 ×10^3 were up-regulated. Conclusion： Detection of spccific protein in human rcnal cell carcinoma scra is significant both for determination of clinical specific biomarkcrs and study of cancer development mechanism.

关 键 词：肾细胞癌 标志物 蛋白质芯片 表面增强激光解吸离子化飞行时间质谱 

分 类 号：R730.3[医药卫生—肿瘤]