槲寄生通过抑制NF-κB活性来诱导人大肠癌HCT116细胞凋亡  

Mistletoe induces apoptosis by down-regulating the constitutive activation of nuclear factor-κB in HCT116 cells

作　　者：林蕾[1] 叶孟[2] 王少敏[2] 倪曙民[2] 吴骁[2] 

机构地区：[1]宁波大学医学院附属医院皮肤科 [2]宁波大学医学院附属医院肿瘤科

出　　处：《中国临床药理学与治疗学》2008年第7期730-734,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：宁波市博士基金资助项目(2005A610020);宁波市医学科研项目(200529)

摘　　要：目的:探讨槲寄生诱导人大肠癌HCT116细胞凋亡所涉及的信号转导途径。方法:采用MTT法测定细胞的增殖活力,琼脂糖凝胶电泳观察细胞调亡,流式细胞仪进行细胞周期分析,Western blotting和DIG-EMSA研究细胞调亡途径。结果:槲寄生对HCT116细胞有明显的生长抑制作用,琼脂糖凝胶电泳检测到典型的DNA梯状条带;槲寄生可通过抑制IκBα蛋白磷酸化进而抑制NF-κB;槲寄生可增强羟基喜树碱对HCT116细胞诱导的凋亡作用。结论:槲寄生对HCT116细胞有明显的生长抑制和诱导调亡作用,并且通过抑制NF-κB活性来诱导人大肠癌HCT116细胞凋亡。AIM： To investigate the role of NF-kB in apoptosis induced by mistletoe in human colon cancer HCT116 cells. METHODS： The cell growth inhibiting was measured by MTF assay, the cell apoptosis was observed by the agarose gel electrophoresis. The protein expression was detected by Western Blotting . DIG-EMSA was conducted to detemine the DNA-binding activation of NF-kB. RESULTS： Mistletoe had a remarkable growth inhibitory action on HCT116 cells. After exposed to mistletoe, HCT116 cells presented some morphologic features of apoptosis including cell shrinkage, nuclear condensation and DNA fragmentation. NF-kB was inhibited in cancer cells treated with mistletoe. Mistletoe may potentiate the cytotoxic effects of chemotherapeutic agents such as HCPF. CONCLUSION： Mistletoe may induce apoptosis in HCT116 cell line. Inhibitory action of NF-kB may play a significant role in apoptosis induced by mistletoe.

关 键 词：槲寄生 大肠癌HCT116细胞系 核转录因子ΚB 凋亡 

分 类 号：R363[医药卫生—病理学]