IFN-γ对MMP-2、-9和TIMP-1在HL-60细胞中表达的影响  

Influence of interferon-γ on the expression of metalloproteinases-2,9 and tissue inhibitor of matrix metalloproteinase-1 mRNA in HL-60 cell line

作　　者：刘心[1] 王婷[1] 杨华[1] 陈葳[1] 徐波[1] 

机构地区：[1]西安交通大学医学院第一附属医院血液科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2008年第4期428-432,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科技攻关基金资助项目(No.2005K10-G1)

摘　　要：目的研究白血病细胞株HL-60中基质金属蛋白酶2、9(MMP-2、MMP-9)和基质金属蛋白酶天然抑制剂-1(TIMP-1)的表达及细胞因子干扰素(IFN-γ)对MMP-2、MMP-9和TIMP-1转录水平的调节。方法分别在HL-60细胞生长的不同时期(1-5d)收集细胞,采用半定量RT-PCR方法,分析白血病细胞株HL-60中MMP-2、MMP-9和TIMP-1mRNA的表达水平;以不同浓度的IFN-γ作用于HL-60细胞,24、48h后RT-PCR检测MMP-2、MMP-9和TIMP-1mRNA水平的变化。结果细胞接种后24hMMP-2和MMP-9的mRNA水平最高,但TIMP-1表达相对恒定。IFN-γ各个浓度组对MMP-2和MMP-9mRNA表达均有抑制作用(P<0.05),且随着作用浓度的增高mRNA表达减少;TIMP-1对其调节不敏感。结论明确了白血病HL-60细胞株在转录水平表达MMP-2、MMP-9和TIMP-1;IFN-γ对MMP-2、MMP-9mRNA表达有明显的抑制作用,而对TIMP-1转录无明显作用。Objective To detect the expression of MMP-2, MMP-9 and TIMP-1 in leukemia cell line HL-60, and explore the effect of interferon- γ （IFN- γ ） on their expression. Methods Reverse transcriptase-polymerase chain reaction was used to assay MMP-2, 9 and TIMP-1 mRNA in leukemia cell line HL-60 at different time （the first day to the fifth day of the cell growth）, and half-quantity values of the influence on the cells after treatment with IFN- γ in different concentration and different time （24 hours, 48 hours）. Results The expression of MMP- 2, -9 mRNA was significantly the highest after 24 hours of the cell culture. But the expression of TIMP-1 mRNA was constant. IFN-γ in different concentration could down-regulate the mRNA expression of MMP-2, -9 （P〈0. 05）, and as the concentration increased, mRNA expression decreased. But mRNA expression of TIMP could not be affected by IFN-γ. Conclusion MMP-2, -9 and TIMP-1 mRNA are expressed in leukemia cell line HL-60. IFN- γ can inhibit expression of MMP-2 or MMP-9, but has no influence on TIMP-1 mRNA expression.

关 键 词：基质金属蛋白酶 基质金属蛋白酶抑制剂 白血病 干扰素-Γ 

分 类 号：R73-3[医药卫生—肿瘤] R733.7[医药卫生—临床医学]