麻黄碱对脑缺血再灌注损伤大鼠脑内星形胶质细胞GFAP表达的影响  

Effects of ephedrine on GFAP of astrocytes after cerebral ischemia-reperfusion injury in rats

作　　者：赵晓科[1] 肖农[2] 张跃[1] 

机构地区：[1]南京医科大学附属南京儿童医院康复科,江苏南京210008 [2]重庆医科大学附属儿童医院神经科,重庆400014

出　　处：《西安交通大学学报（医学版）》2008年第4期465-467,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：重庆市医学科技计划基金资助项目(渝中医2003No.32)

摘　　要：目的探讨大鼠脑缺血再灌注损伤后麻黄碱治疗对星形胶质细胞胶质原纤维酸性蛋白(GFAP)表达水平的影响。方法雄性SD大鼠60只,随机分为假手术组、自然恢复组和治疗组。应用线栓法建立单侧大脑中动脉缺血再灌注模型,术后1、2、3、4周应用免疫组化观察缺血周围区GFAP的表达。结果自然恢复组和治疗组术后1周GFAP表达开始增加,3周后表达平稳。治疗组GFAP的表达水平在前两个时间点高于自然恢复组(P<0.05)。结论麻黄碱对脑缺血再灌注损伤大鼠缺血周围区星形胶质细胞增殖活化有促进作用。Objective To investigate the effects of ephedrine on glial fibrillary acidic protein （GFAP） expression after cerebral ischemia-reperfusion injury in rats. Methods Altogether 60 male SD rats were randomly divided into sham-operation group, natural recovery group and ephedrine treatment group. The unilateral ischemia-reperfusion models were induced by clue-blocked method. The expression level of GFAP around ischemic area was examined by immunohistochemical technique at weeks 1, 2, 3 and 4 after operation. Results GFAP expression began to increase at 1 w and stabilized at 3 w in ephedrine treatment group and natural recovery group. There was a significant increase of GFAP expression in ephedrine treatment group compared with that in natural recovery group （P 〈 0. 05） at the first two time points. Conclusion Ephedrine treatment facilitates the activation and proliferation of astrocytes induced by cerebral ischemia-reperfusion injury.

关 键 词：麻黄碱 脑缺血再灌注 星形胶质细胞 胶质原纤维酸性蛋白(GFAP) 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学] R285[医药卫生—临床医学]