云南小麦高分子量麦谷蛋白亚基基因1Dy12^＊的分离  

Isolation of High Molecular Weight Glutenin Subunit Gene 1Dy12^＊ from Yunnan Wheat （Triticum aestivum subsp, yunnanense King）

作　　者：代小华[1] 房敬业[1] 李宗瑾[1] 刘勇[1] 邹珂[1] 汪越胜[1] 常俊丽[1] 何光源[1] 

机构地区：[1]华中科技大学生命科学与技术学院,分子生物物理教育部重点实验室,武汉430074

出　　处：《武汉植物学研究》2008年第4期325-328,共4页Journal of Wuhan Botanical Research

基　　金：国家973计划项目资助课题(2002CB111301)

摘　　要：通过SDS-PAGE分析,从云南小麦中鉴定出一个电泳迁移率比高分子量麦谷蛋白亚基1Dy12稍快的亚基1Dy12*。利用Glu-Dy位点特异引物对1Dy12*基因编码区进行了克隆和序列测定。1Dy12*基因全长为1980bp,编码658个氨基酸。氨基酸序列比较结果表明:与亚基1Dy12相比有3个氨基酸的差异和1个二肽(GQ)的缺失,与亚基1Dy10相比有15个氨基酸的差异、2个六肽(IGQGQQ)的插入以及1个二肽(GQ)的缺失。这表明1Dy12*亚基是一个新型高分子麦谷蛋白亚基,其对小麦加工品质的影响正在评价中。A novel y-type high molecular weight glutenin subunit （HMW-GS） possessing a slightly faster mobility than that of subunit 1Dy12 was identified through SDS-PAGE analysis, designated 1Dy12^＊ in Yunnan wheat （ Triticum aestivum subsp, yunnanense King）. The coding region of HMW-GS gene 1Dy12^＊ was isolated and sequenced with Glu-Dy locus specific primers. The complete open reading frame （ORF） of 1Dy12^＊ was 1980 bp,encoding 658 amino acids. Comparison of amino acid sequences showed there were 3 amino acid differences between subunits 1Dy12^＊ and 1Dy12 and a deletion of dipeptide （GQ） in subunit 1 Dy12^＊. Also, there were fifteen amino acid differences between subunits 1Dv12^＊ and 1Dv10. two hexapeptides （IGQGQQ） insertions and a deletion of dipeptide （GQ） in subunit 1Dy12^＊. This showed that subunit 1Dy12^＊ is a novel HMW-GS, and it is in process of evaluating subunit 1Dy12^＊ to effect wheat processing quality.

关 键 词：云南小麦 高分子量麦谷蛋白亚基 序列分析 

分 类 号：Q946.1[生物学—植物学]