固氮菌B8-G菌株产聚-β-羟基丁酸发酵条件研究  

The Study of the Culture Condition of B 8 G Strain ( Azotobacter ) Produced Poly β Hydro Butyric Acid

作　　者：董兆麟[1] 史秀杰[1] 

机构地区：[1]西北大学生物学系

出　　处：《西北大学学报（自然科学版）》1997年第5期448-451,共4页Journal of Northwest University（Natural Science Edition）

基　　金："九五"期间国家"863计划"重点资助

摘　　要：通过利用碳源、氮源实验，发现固氮菌Ｂ８－Ｇ菌株，在以ＮＨ４ＮＯ３为氮源、蔗糖或甘露醇为碳源的发酵培养基中生长良好。在３０℃，２２０ｒｐｍ摇床培养４８ｈ，Ｂ８－Ｇ菌株的生物量可达９．５１ｇ·Ｌ－１以上，ＰＨＢ含量可达细胞干重的７４％以上。同时对Ｂ８－Ｇ菌株的培养条件也作了研究，采用补料分批培养方法，获得Ｂ８－Ｇ菌株的生物量达到１７．２ｇ·Ｌ－１；ＰＨＢ的含量达到７６．１４％。By the tests of utilization of carbbon souce and nitrogen souce, it was found that the B 8 G strain ( Azotobacter ) can grow well in the medium, using NH 4NO 3 as nitrogen source and sucrose or mannitol as carbon source. The culture was cultivated for 48 h under the condition of 32℃ and 220 rpm. The biomass of B 8 G could reach 9.51 g/l, the content of PHB was more than 74%. The orther culture condition of B 8 G strain was also studied. By the method of supplemented batch cultivation, the high biomass of B 8 G was obtained which was 17.2 g/l, and PHB content reached 76.14% of cell dry weight.

关 键 词：固氮菌B8-G菌株 发酵 生物降解塑料 聚羟基丁酸 

分 类 号：TQ324.9[化学工程—合成树脂塑料工业]