死亡相关蛋白激酶基因在胃癌组织中的表达及其临床意义  

The expression of death-associated protein kinase in primary gastric cancer

作　　者：叶梅[1] 夏冰[2] 李冬青[3] 周峰[2] 郭秋莎[2] 刘铭球[4] 

机构地区：[1]武汉大学中南医院综合科,武汉430071 [2]武汉大学消化系病研究中心,武汉430071 [3]武汉大学医学院免疫学系 [4]武汉大学医学院病理学系

出　　处：《临床内科杂志》2008年第8期555-558,共4页Journal of Clinical Internal Medicine

基　　金：湖北省科技攻关项目(2004AA301C91);湖北省自然科学基金项目(2007ABA261)

摘　　要：目的研究死亡相关蛋白激酶(DAPK)基因在原发性胃癌组织中的表达及其与临床病理特征的关系,并探讨其在胃癌发生发展中可能的机制。方法收集62例原发性胃癌及相对应的癌旁正常组织。应用RT-PCR法检测DAPK mRNA的表达,应用Western-blot及免疫组化方法检测DAPK蛋白的表达。结果DAPKmRNA和蛋白表达水平在胃癌组织中明显低于癌旁正常组织(OD值分别为0.2863±0.2027 vs 0.5736±0.1968,P<0.0001;0.2616±0.0913 vs 0.6529±0.1808,P<0.0001)。免疫组化分析显示,DAPK在所有癌旁正常组织中表达均为阳性,均值为8.61±2.89;而在胃癌组织中表达则明显减弱或缺失,均值为2.90±3.38。DAPK在癌旁正常组织中的表达水平显著高于胃癌组织(P<0.0001)。DAPKmRNA及蛋白表达水平与胃癌分期和淋巴结转移显著相关。结论在原发性胃癌组织中,DAPKmRNA和蛋白表达明显缺失或低下,且与胃癌临床分期和淋巴结转移显著相关。Objective To investigate the expression of DAPK, an apoptosis-related serine/threonine kinase gene,in tissue of primary gastric cancer （GC） at mRNA and protein levels and to evaluate the clinical implication. Methods DAPK mRNA expression was determined using RT-PCR, and the protein expression was measured using immunohistochemical staining in 62 GC tissues and adjacent normal tissues. The expression of DAPK protein level was further determined using Western-blotting. Results DAPK mRNA and protein expressions by Western blotting in GC were reduced significantly than those in corresponding normal tissues. Immunohistochemical staining revealed that expression of DAPK was significantly lower in GC than that in matched normal tissues. Furthermore, there was a significant correlation between DAPK mRNA and protein expressions, stage classification and lymph node metastasis of GC. Conclusion Expression of DAPK was significantly reduced in tissue of GC at mRNA and protein levels, and the expression was related to the stage classification and lymph node metastasis of GC.

关 键 词：死亡相关蛋白激酶 基因表达 胃肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]