Caspase-9在结直肠正常黏膜和癌中的表达  

Expression of Caspase-9 gene in colorectal normal mucosa and cancer

作　　者：沈小钢[1] 周总光[2] 王存[1] 王铃[1] 周兵[1] 

机构地区：[1]四川大学华西医院普外三科,成都市610041 [2]四川大学华西医院消化外科实验室、普外科,成都市610041

出　　处：《实用医学杂志》2008年第17期2978-2980,共3页The Journal of Practical Medicine

摘　　要：目的:定量测定Caspase-9基因在人类结直肠癌及癌旁正常组织中的表达,从而探讨其在结直肠癌发病中的作用。方法:采用实时荧光定量PCR技术测定88例配对的结直肠癌组织及癌旁正常组织中Capase-9 mRNA的表达,并分析表达的变化与临床病理特征的关系。结果:Caspase-9在结直肠癌中相对于癌旁正常组织表达下调(P=0.003)。结直肠癌组织分化程度与Caspase-9表达下调相关(P=0.048),与Dukes分期、年龄、性别及是否淋巴结转移无关。结论:Caspase-9在结直肠癌的发病中可能发挥了重要作用,并参与了肿瘤组织的分化。Objective To quantitatively measure the expression of Caspase-9 gene in human colorectal cancer and near-cancer normal mucosa so as to investigate the proper role of it in carcinogenesis in colorectal cancer. Methods Eighty-eight paired samples including near-cancer colorectal normal mucosa and matched cancerous tissue were included. Caspase-9 mRNA was detected by real-time PCR. Results Compared with near-cancer colorectal normal mucosa, the expression of Caspase-9 mRNA in cancerous tissue was lower （P = 0.003）. The difference in expression of Caspase-9 mRNA was significant for cancer of different differentiation （P = 0.048）, while not for Dukes stage, age, sex and lymph node status. Conclusion Compared to near-cancer colorectal normal mucosa, the expression of Caspase-9 is lower in cancer tissue, and is also related to cancer differentiation.

关 键 词：结直肠肿瘤 CASPASE-9 实时荧光定量PCR 

分 类 号：R735.3[医药卫生—肿瘤]