紫杉醇纳米微粒对人胃癌细胞MGC803增殖和凋亡影响的研究  

Effect of Paclitaxel-loaded Microspheres on Proliferation and Apoptosis of Human Gastric Carcinoma Cell Lines MGC803

作　　者：石艳会[1] 费洪新[2] 卢长柱[2] 孙丽慧[2] 郭志鸿[3] 

机构地区：[1]齐齐哈尔市第一医院,黑龙江齐齐哈尔161005 [2]齐齐哈尔医学院,黑龙江齐齐哈尔161042 [3]中国科学院寒区旱区环境与工程研究所,兰州730070

出　　处：《中国现代医生》2008年第23期22-24,共3页China Modern Doctor

基　　金：黑龙江教育厅2007年科学技术研究项目(11521323)

摘　　要：目的观察不同浓度(紫杉醇)PA纳米微粒在不同时间段对人胃癌MGC803细胞生长和凋亡的影响。方法应用MTT法测定不同浓度的PA纳米微粒、PA和5-FU对MGC803细胞分别在0、6、12、24、48、60、72h的细胞生长抑制率。流式细胞仪分析MGC803细胞周期的改变,细胞免疫组化检测凋亡相关基因Bcl-2蛋白的表达,检测端粒酶活性。结果MTT法显示不同浓度的PA纳米微粒可抑制MGC803细胞增殖(16μg/mL作用72h时的抑制率达69%),并且与浓度和时间均呈正相关,流式细胞仪细胞周期分析提示PA纳米微粒可将MGC803细胞阻滞于G2M期,16μg/mL作用48h明显,细胞免疫组化法显示凋亡相关基因Bcl-2蛋白表达下调,端粒酶活性水平下降。结论PA纳米微粒可诱导人胃癌细胞MGC803发生凋亡且具有控释效应,可延长药物对胃癌细胞的有效作用时间,载药纳米微球没有改变PA成分的生物学活性。Objective To observe the effects of paelitaxel-loaded mierospheres with magnetic nanoparticles on proliferation and apoptosis of human gastric carcinoma cell line MGC803. Methods Methyl thiazolyl tetrazolium assay was used to establish the dose-effect curves. Flow cytometry analysis was used to observe the cell cycle, hnmunohistochemical staining was used for detecting expression status of apoptosis-related Bcl-2 protein,the effect measured telomerase. Results Paclitaxel-loaded microspheres with magnetic nanoparticles could inhibit the pro- liferation of in vitro（ 16 μL g/mL for 72h showed 69% ）,which was associated with arrest of G2M phase, 16 μg/mL for 48h showed,and showed time-dependent and level-dependent manner, hnnmnohistochemical staining showed a decreased expression of Bcl-2 protein of MGC803 cell fine after incubation with Paelitaxel-loaded microspheres,and inhibited telomerase activity. Conclusion Paclitaxel-loaded mierospheres with magnetic nanoparticles is able to induce the apoptosis in human gastric carcinoma cell MGC803 and has drug sustained release character, which can prolong the phase of inhibitory effect of paclitaxel against gastric carcinoma cells. The paclitaxel-loaded nanoparticles do not reduce the biological activity of paclitaxel.

关 键 词：紫杉醇 胃癌 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]