改构型酸性成纤维细胞生长因子对大鼠动脉血栓形成的影响  

Effect of Modified Acidic Fibroblast Growth Factor on Thrombosis of Artery in Rat

作　　者：黄彬[1] 黄巨恩[1] 胡世凤[2] 李校坤[3] 肖健[3] 

机构地区：[1]广西医科大学护理学院,广西南宁530021 [2]广西医科大学生理教研室,广西南宁530021 [3]温州医学院生物与天然药物研究院,浙江温州325027

出　　处：《解剖科学进展》2008年第3期260-262,共3页Progress of Anatomical Sciences

基　　金：国家"863"计划资助项目(2004AA2Z3C60)

摘　　要：目的探讨改构型酸性成纤维细胞生长因子(modified acidic fibroblast growth factor,MaFGF)对大鼠颈总动脉血栓形成的影响。方法Wistar大鼠50只,随机分成生理盐水组、肝素组、MaFGF组(又分MaFGF1组、MaFGF组、MaFGF组),每组10只。于大鼠的股静脉分别注射生理盐水(0.5ml/100g)、肝素钠(0.1U/10023g),MaFGF1组(10U/100g)、MaFGF2组(100U/100g)、MaFGF3组(1000U/100g),给药后5min,用保护电极刺激颈总动脉,直至血压曲线逐渐消失,记录刺激开始至压力曲线消失的时间,即血栓堵塞时间。结果肝素组、MaFGF2组、MaFGF3组与生理盐水组比较,平均堵塞时间延长(P<0.05或P<0.01);肝素组与各MaFGF组之间比较,肝素的平均堵塞时间明显较长(P<0.001)。结论MaFGF可以延长大鼠动脉血栓形成的时间,并呈现一定的量效关系。Objective To explore the influence of Modified acidic fibroblast growth factor（MaFGF） on thrombosis in rats＇ common carotid. Method 50 wistar rats were divided averagely into saline, heparin and MaFGF groups （MaFGF1, MaFGF2 and MaFGF3）. Saline, heparin sodium（0.1U/100g）and MaFGF （10U, 100U, 1000U/100g） were injected into rat＇s femoral veins respectively, min later, electric stimulations were carried out below inside-putting tubes of artery, keeping to the curvilinear disappearance of blood pressure and then recording the time from electric stimulation to the pressure curve disappearance （namely the blood clot occluding time, BCOT）. Result The average BCOT was longer in Heparin, MaFGF2 and MaFGF3 comparing with Saline group, （P〈0.05 or P〈0.01）; showed that the average BCOT was obviously longer in heparin group than in MaFGF groups（P〈0.001） Conclusion MaFGF could prolong the time of rat＇s thrombosis of artery with certain dosage-effect relationship.

关 键 词：改构型酸性成纤维细胞生长因子 血栓形成 颈总动脉 大鼠 

分 类 号：R743.32[医药卫生—神经病学与精神病学]