喉癌中EGFR基因扩增、表达及临床意义  

EGFR Gene Amplification ,Overexpression and its Clinical Implications in Laryngeal Squamous Cell Carcinoma

作　　者：李海洲[1,2] 李巍[3] 米玉录[1] 王旭红[1] 

机构地区：[1]山西医科大学山西省肿瘤医院头颈科 [2]锦州市中心医院,辽宁锦州121000 [3]锦州市中心医院

出　　处：《解剖科学进展》2008年第3期302-304,共3页Progress of Anatomical Sciences

摘　　要：目的研究喉癌中表皮生长因子受体(EGFR)基因的扩增、表达,探讨其在喉癌发生、发展中的作用及临床意义。方法采用差异PCR(differential PCR)方法检测40例喉鳞状细胞癌及配对癌旁正常组织中EGFR基因的扩增(即基因拷贝数增加);应用RT-PCR方法检测EGFR mRNA水平;应用SPSS13.0软件对数据进行统计学分析。结果喉癌组织中有13例(占32.5%)EGFR基因拷贝数增加,癌旁对照组中则未检测到(χ2=15.537,P<0.005);喉癌组织中EGFR mRNA平均积分光密度为872.356±62.340,癌旁对照组为346.425±57.380(t=5.959,P<0.001);喉癌组织分化程度越低,病理分期越晚,EGFR基因扩增和mRNA表达水平越高(P<0.05)。结论喉癌中EGFR基因在DNA水平上的扩增是EGFR mRNA过表达的原因之一,EGFR的扩增和过表达在喉癌的发生、进展中发挥一定作用。Objective To detect the amplification and overexpression of epidermal growth factor receptor （EGFR） gene in laryngeal squamous carcinoma and their clinical significance. Methods EGFR gene amplification was detected by differential PCR approach in 40 cases. EGFR mRNA of carcinoma tissues and normal tissues was evaluated by reverse transcription polymerase chain reaction（RT-PCR）. Statistical analysis was performed using SPSS13.0. Results DNA sequence copy was increased in 13 （32.5%）cases of laryngeal carcinoma, but negative in paired normal tissue （ X^2=15.537, P〈 0.001）. The mean integrated optical density of EGFR was 872.356 ± 62.340 in laryngeal carcinoma tissue, 346.425± 57.380 in the paired normal tissue （t= 5.959 ,P〈0.05）. There was a significant correlation of EGFR amplification and mRNA overexpression with advanced clinical pathological stage,and also with poor differentiated degree of cancer cells. Conclusion EGFR gene amplification contributes to its mRNA overexpression , EGFR gene may play an important role in laryngeal carcinoma oncogenesis and malignant progression.

关 键 词：喉鳞状细胞癌 EGFR基因 差异PCR 基因扩增 

分 类 号：R739.65[医药卫生—肿瘤]