己酮可可碱对非酒精性脂肪性肝炎大鼠肝组织MMP-9、TGFβ1表达的影响  

Effect of Pentoxifylline on the Expressions of MMP-9 and TGFβ1 in Hepatic Tissue of Rats with Nonalcoholic Steatohepatitis

作　　者：任燕龙[1] 孙海梅[2] 曾晓蓓[2] 尚宏伟[2] 张华[2] 安威[3] 

机构地区：[1]首都医科大学2004级临床医学专业七年制2班 [2]首都医科大学基础医学院组织胚胎学教研室 [3]首都医科大学基础医学院肝脏保护与再生调节实验室

出　　处：《首都医科大学学报》2008年第4期463-466,共4页Journal of Capital Medical University

基　　金：北京市教育委员会科技发展计划(KM200710025003)资助项目~~

摘　　要：目的研究己酮可可碱(PTX)对非酒精性脂肪性肝炎(NASH)肝组织基质金属蛋白酶9(MMP-9)、转化生长因子β1(TGFβ1)表达的影响。方法高脂饮食法建立大鼠NASH模型,取SD大鼠40只,分为对照组、12周模型组、16周模型组和PTX治疗组,每组10只。采用改良Masson三色染色检测肝纤维化面积,免疫组织化学方法检测肝组织MMP-9、TGFβ1的表达以及分布。结果免疫组织化学方法检测结果显示,与对照组相比,12周、16周模型组和PTX治疗组肝组织MMP-9的表达量明显升高(P<0.05);16周模型组和PTX治疗组TGFβ1的表达量亦明显升高(P<0.05);与16周模型组相比,PTX治疗组TGFβ1表达量显著降低(P<0.05)。检测肝纤维化面积,12周、16周模型组和PTX治疗组的面积均大于对照组(P<0.05)。且16周模型组面积大于12周模型组(P<0.05)。PTX治疗组面积较16周模型组下降(P<0.05)。结论PTX可降低TGFβ1的表达,改善肝纤维化面积,提示PTX对实验性NASH具有治疗作用。PTX对MMP-9的表达无影响。Objective To investigate the effects of pentoxifylline （ PTX ） on the expressions of metalloproteinase-9 （ MMP-9 ） and transforming growth factor β1 （TGF β1 ） in hepatic tissue of rats with nonalcoholic steatohepatitis （NASH）. Methods Rat model of NASH was established by giving a fat-rich diet. Forty Sprague-Dawley rats were randomly divided into four groups, control group（ n = 10 ）, 12 week model group （n = 10 ） , 16 week model group（ n = 10 ） and PTX-intervention group （ n = 10 ）. The area of liver fibrosis was determined by Masson staining. The expression of MMP-9 and TGFβ1 were detected by immunohistochemistry. Results The results of immunohistochemical staining shown that the expression of MMP-9 was significantly higher in rats of model groups and PTX-intervention group than control group （P 〈0.05 ） ; The expression of TGFβ1 was higher in rats of 16 week model group and PTX-intervention group than control group（ P 〈 0.05 ） ; Comparing with the 16 week model group, the expression of TGFβ1 in PTX-intervention group decreased significantly（ P 〈0.05 ）. The fibrosis area of masson staining was higher in rats of model groups and PTX-intervention group than control group（P 〈 0.05 ）; The area in 16 week model group was higher than 12 week model group（ P 〈 0.05 ）; Comparing with the 16 week model group, the area in PTX-intervention group decreased significantly （ P 〈 0.05 ）. Conclusion PTX interventional reduced the content of TGFβ1, while that of MMP-9 expression was unchanged. These may improve the degree of hepatic fibrosis. PTX may be useful in the treatment of NASH and liver fibrosis.

关 键 词：非酒精性脂肪性肝炎 己酮可可碱 肝纤维化面积 基质金属蛋白酶9 转化生长因子Β1 

分 类 号：R575[医药卫生—消化系统]