视神经挫伤对大鼠视网膜谷氨酰胺合酶和兴奋性氨基酸转运蛋白-2表达的影响  

Optical nerve contusion on expression of glutamine synthetase and GLT-1 in rat retina

作　　者：黄正如[1] 陈海英[1] 徐明[1] 陆炯[1] 叶凯[1] 管怀进[2] 

机构地区：[1]江苏省常熟市第二人民医院眼科,215500 [2]南通大学附属医院眼科,江苏省南通市226001

出　　处：《眼科新进展》2008年第9期657-660,共4页Recent Advances in Ophthalmology

摘　　要：目的通过谷氨酰胺合酶(glutamine synthetase,GS)、兴奋性氨基酸转运蛋白-2(GLT-1)表达的改变,研究视神经挫伤后视网膜谷氨酸代谢变化的机制。方法建立大鼠右眼视神经挫伤模型48只。术后1d、7d、14d以高效液相色谱分析检测大鼠玻璃体谷氨酸浓度;通过免疫组织化学检测大鼠视网膜GS、GLT-1的表达。结果视神经挫伤后1d、7d、14d,大鼠玻璃体谷氨酸浓度升高,与对侧眼比较差异具有统计学意义(P=0.0011、P=0.0000、P=0.0000)。视神经挫伤后1d,GS高表达(P=0.0054);挫伤后7d,GS表达与对侧眼比较差异无统计学意义(P=0.1379);挫伤后14d,GS低表达(P=0.0333)。视神经挫伤后1d、7d,GLT-1的表达与对照组的差异无统计学意义(P=0.1985、0.6537);但挫伤后14d,GLT-1低表达(P=0.0403)。结论视神经挫伤后玻璃体谷氨酸浓度升高,与视网膜GS、GLT-1的表达降低有关。Objective To investigate the expression of glutamine synthetase（GS） and GLT-1 in rat retina and mechanisms of retinal glutamic acid metabolism after optical nerve contusion. Methods Forty-eight Sprauge-Dawley（SD） rats were contused right optical nerve. Vitreous glutamate concentration was analyzed with high-performance liquid chromatography at 1 day, 7 days, 14 days post-contusion. The expression of GS and GLT-1 were measured by means of immunohistochemistry. Results The vitreous glutamate concentration of right eyes was higher than that of left eyes at 1 day,7 days,14 days post-contusion （P = 0. 001 1 ,P = 0. 000 0 ,P=0. 000 0 ）. GS expression of right eyes was increased at 1 day post-contusion （ P =0. 005 4 ） ,but was decreased at 14 days post- contusion（P= 0. 033 3 ）, and with no significant difference comparing with left eye at 7 days post-contusion（ P = 0. 137 9 ）. GLT-1 expression of right eyes also decreased at 14 days post-contusion（ P = 0. 040 3 ）, and with no statistical differ- ence comparing with left eye at 1 day and 7 days post-contusion （ P =0. 198 5 ,P = 0.653 7 ）. Conclusion Optical nerve contusion could decrease the expression of GS and GLT-1 in retina, which results in the increase of glutamate concentration.

关 键 词：视神经挫伤 谷氨酸 谷氨酰胺合酶 兴奋性氨基酸转运蛋白-2 

分 类 号：R774.6[医药卫生—眼科]