16S rRNA/DNA-PCR对初生新生儿肠道乳酸杆菌和大肠埃希菌的动态检测  

Study on the variation of Lactobacillus and Escherichia coli in earlier newborn babies by fluorescent quantitative 16S rRNA/DNA targeted PCR

作　　者：杨武[1] 刘华[2] 曹志琅[3] 梁琨[1] 李利[3] 黄永坤[1] 贺湘英[1] 李海林[1] 

机构地区：[1]昆明医学院第一附属医院儿科,云南昆明650032 [2]昆明医学院第一附属医院检验科基因研究室,云南昆明650032 [3]云南省第一人民医院儿科,云南昆明650032

出　　处：《中国微生态学杂志》2008年第4期356-358,共3页Chinese Journal of Microecology

基　　金：云南省中青年学术技术带头人后备人才培养计划资助项目(2006py01-18)

摘　　要：目的观察新生儿生后肠道菌群的动态变化。方法采用16S rRNA/DNA荧光定量PCR技术,分别对40例足月儿和40例早产儿生后第1(>12h)、4、7天粪便标本中的乳酸杆菌和大肠埃希菌进行定量分析。结果不同日龄足月儿粪便标本中乳酸杆菌数量的对数值分别为5.50±0.81、6.87±0.81、9.20±0.87,早产儿分别为4.89±0.46、6.05±0.46、8.06±0.18;不同日龄足月儿粪便标本中大肠埃希菌数量的对数值分别为6.49±0.40、7.59±0.58、7.15±0.55,早产儿分别为6.32±0.51、7.39±0.78、7.05±0.63;2种细菌对数值分别在组间行单因素方差分析,差异有显著性(P<0.05);组内行配对t检验,差异也有显著性(P<0.05)。结论新生儿肠道菌群的建立是动态变化的,个体间差异受多因素影响,早产儿落后于足月儿。Objective To observe the variation of intestinal flora in newborn babies. Methods The amount of stool Lactobacillus and Escherichia coli of first day, fourth day, seventh day after birth in 40 term and 40 preterm babies were measured by the practical fluorescent quantitative 16S rRNA/DNA-targeted PCR. Results Lactobacillus logarithmic abso- lute value of first day, fourth day, seventh day after birth were 5.50 ± 0.81,6.87 ± 0.81,9.20 ± 0.87 in term babies re- spectively, and 4.89 ± 0.46,6.05 ± 0.46,8.06 ±0.18 in preterm babies respectively. Escherichia coli logarithmic absolute value of first day, fourth day, seventh day after birth were 6.49 ± 0.40,7.59 ± 0.58,7.15 ± 0.55 in term babies respective- ly, and 6.32 ±0. 51 ,7.39± 0. 78,7.05 ± O. 63 in preterm babies respectively. The above values had statistical difference between group-to-group and within group （ P 〈 0.05 ）. Conclusions There are developmental variation of intestinal flora formation in newborn babies. The intestinal bacterial flora formation of the preterm babies is slower than that of tenn.

关 键 词：新生儿 肠道菌群 荧光定量 PCR 

分 类 号：R575[医药卫生—消化系统]