脑微血管内皮细胞培养技术的研究进展  

Advance in study of the culture of brain microvascular endothelial cells

作　　者：赵琳[1] 郭虹[1] 康立源[1] 胡利民[1] 

机构地区：[1]天津中医药大学中医药研究院,天津市中药药理学实验室,省部共建教育部方剂学重点实验室,天津300193

出　　处：《中国药理学通报》2008年第8期992-995,共4页Chinese Pharmacological Bulletin

基　　金：国家重点基础研究发展计划(973计划)项目(No2005CB523404);国家自然科学基金资助项目(No30572348)

摘　　要：脑微血管内皮细胞作为构成血脑屏障(blood brain barrier,BBB)的主要结构基础,由于其特殊的细胞膜转运系统和细胞间的紧密连接,从而在中枢神经系统中的血管内外物质交换中发挥着重要的作用。因其与脑缺血、脑水肿等脑血管疾病的病理过程有着密切的关系,因此体外培养脑微血管内皮细胞(brain microvascular endothelial cells,BMECs)为研究脑血管疾病提供了一种新的方法。该文通过对国内外相关文献进行检索,综合了国内外近年来的研究,依次从大鼠脑微血管内皮细胞的分离、纯化、培养等几个方面,探讨了脑微血管内皮细胞的体外培养技术,为脑微血管内皮细胞的体外研究提供基础。Blood-brain barrier （BBB） is mainly composed of brain microvascular endothelial cells （BMECs）. According to the special cell membrane transport system and the extensive tight junctions,the brain microvascular endothelial cells play an important role in the CNS. Also, there is a close relationship be- tween BMECs and the cerebrovascular diseases, such as cerebral ischemia,cerebral edema, and so on. Therefore, the culture of brain microvascular endothelial cells provides a new tool for studying the cerebrovascular diseases in vitro. The researches in recent years have been reviewed in this test ,approaching the cul- tivation of the rat brain microvascular endothelial cell from the segregation, purification and culture in turns, in order to provide a foundation for the studies of the brain microvascular endothelial cell ; in vitro.

关 键 词：脑 微血管内皮细胞 细胞培养 

分 类 号：R-05[医药卫生] R322.12