青藤碱对吗啡依赖小鼠脊髓与小脑中HO2、sGCα1和sGCα2基因mRNA表达的影响  

Effect of sinomenine on the expression of HO2,sGCα1 and sGCα2 genes of spinal cord and cerebellum in morphine dependence mice

作　　者：郑济芳[1] 刘珍[1] 朱允华[1] 刘俊[1] 胡弼[1] 

机构地区：[1]南华大学生命科学与技术学院,湖南衡阳421001

出　　处：《中国药理学通报》2008年第8期1044-1048,共5页Chinese Pharmacological Bulletin

基　　金：湖南南华大学博士启动基金资助项目(No5-03-XJQ-03-044);湖南省自然科学基金资助项目(No04JJ30046)

摘　　要：目的评价青藤碱治疗前后吗啡依赖与戒断小鼠脊髓和小脑中HO2、sGCα1和sGCα2基因mRNA水平的变化,探讨青藤碱作用于吗啡依赖小鼠的分子机制。方法采用吗啡剂量递增法,建立小鼠吗啡依赖模型,用青藤碱(40mg.kg-1,ip)治疗吗啡依赖小鼠后,再用纳洛酮激发急性戒断症状,半定量RT-PCR测脊髓和小脑HO2、sGCα1和sGCα2基因的mRNA水平变化。结果慢性吗啡用药使小鼠脊髓与小脑中HO2和sGCα1基因的mRNA水平发生异常上调,sGCα2基因的mRNA水平没有异常上调。单独使用青藤碱,没有引起小鼠脊髓与小脑中这些指标异常升高。青藤碱治疗后,小鼠吗啡依赖与戒断时在脊髓与小脑中异常上调的HO2和sGCα1基因的mRNA水平下降到接近对照组水平。结论慢性吗啡用药使HO2和sGCα1基因的mRNA水平发生不同程度的异常上调,这种上调在青藤碱治疗后可以恢复到接近正常水平,这可能是青藤碱对吗啡依赖小鼠治疗作用的分子机制之一。Aim To explore the molecular mechanism of Sinomenine on the morphine dependence mice, and to evaluate the mRNA levels of HO2, sGCα1 and sGCα2 genes were evaluated in the spinal cord and cerebellum tissues from the morphine dependence mice. Methods Mice were subjected to injection of morphine with an increasing dose for five days, and then were treated with Sinomenine （40 mg · kg-1,ip） for another five days. Naloxone was used to develop a- cute withdrawal. The mRNA levels of HO2 sGCα1 and sGCα2 genes in the spinal cord and cerebellum tissues were determined by semi-quantitative RT-PCR. Results After exposure to morphine chronically, the mRNA levels of HO2 and sGCα1 genes were increased obviously. The mRNA level of sGCα2 gene was not in- creased obviously. Only administration of sinomenine alone could not alter the mRNA levels of HO2, sGCα1 and sGCα2 genes. Sinomenine could reduce the up- regulation of mRNA levels of HO2 and sGCα1 genes. Conclusions The mRNA expressions of HO2 and sGCα1 genes were increased after chronic morphine administration and could be regulated by sinomenine treatment. This might be one of the molecular mechanisms in which sinomenine was treated on the morphine dependence mice.

关 键 词：青藤碱 吗啡 血红素加氧酶2 可溶性鸟苷酸环化酶 

分 类 号：R-332[医药卫生] R284.1