大鼠脑缺血/再灌注后神经元凋亡及caspase-12 mRNA和蛋白表达的改变  

Neuronal apoptosis and expression of caspase-12 mRNA and protein following focal cerebral ischemia-reperfusion in rats

作　　者：张鸿[1] 宋利春[1] 贾春红[1] 刘艳艳[2] 吕永利[3] 

机构地区：[1]中国医科大学附属盛京医院神经内科,辽宁沈阳110004 [2]天津市天和医院,天津300050 [3]中国医科大学基础医学院解剖学教研室,辽宁沈阳110001

出　　处：《中国药理学通报》2008年第8期1069-1072,共4页Chinese Pharmacological Bulletin

基　　金：辽宁省自然科学基金资助项目(No20052097)

摘　　要：目的研究大鼠局灶性脑缺血/再灌注后caspase-12 mRNA及蛋白的表达,明确内质网信号通路在脑缺血后神经元凋亡调控中的作用。方法60只Wistar♂大鼠随机分为假手术组和缺血组。应用线栓法制备大鼠大脑中动脉缺血/再灌注模型,应用原位末端标记法(TUNEL)、免疫组化染色、RT-PCR技术检测脑缺血/再灌注后各组凋亡细胞数和caspase-12 mRNA及蛋白的表达。结果脑缺血/再灌注后,随着再灌注时间的延长,凋亡细胞数和caspase-12mRNA及蛋白的表达逐渐增高。在脑缺血/再灌注后24h达高峰。与假手术组相比,缺血组在各时间点凋亡细胞数、caspase-12 mRNA及蛋白表达均明显升高(P<0.01)。结论脑缺血/再灌注后神经元凋亡数明显增高,caspase-12 mRNA及蛋白的表达明显升高,提示内质网通路参与了脑缺血/再灌注后神经元凋亡的调控。Aim To study the expression of caspase-12 mRNA and protein following focal cerebral ischemia- reperfusion in rats, and explore the effect of endoplas- mic reticulum pathway on neuronal apoptosis. Methods 60 male Wistar rats were randomly divided into sham-operated group and ischemic group. The middle cerebral artery occlusion （MCAO） models were estab- lished by using the intraluminal suture occlusion meth- od, neuronal apoptosis was detected by TUNEL stai- ning,the expression of caspase-12 protein was detected by immunohistochemical staining, the expression of caspase-12 mRNA was detected by RT-PCR method. Results In ischemic group, the number of apoptotic cells and the expression of caspase-12 mRNA and protein were gradually increased following prolonged cere- bral reperfusion, reached the peak at 24 h. The number of apoptotic cells and the expression of caspase-12 mR- NA and protein in ischemic group were significantly less than those of sham-operated group at all times （P 〈0. 01 ）. Conclusion The number of apoptotic cells and the expression of caspase-12 mRNA and protein increased significantly following focal cerebral ischemi- a-reperfusion, which indicated endoplasmic reticulum pathway might be involved in regulating neuronal apoptosis following cerebral ischemia-reperfusion.

关 键 词：脑缺血 凋亡 CASPASE-12 内质网 

分 类 号：R-332[医药卫生] R322.81