Arylamine N-acetyltransferases: a new inhibitor of apoptosis in HepG2 cells  

作　　者：Yu-bin JI Shi-yong GAO 

机构地区：[1]Postdoctoral Research Station of the Institute of Materia Medica, Center for Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China [2]MOE Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin 150076, China

出　　处：《浙江中西医结合杂志》2008年第9期701-706,共6页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：the National Natural Science Foundation of China (No. 30400591);the Science Foundation of Heilongjiang Province, China (Nos. D2004-13 and D200505);the Young Scientist Fund of Harbin City, China (No. 2004AFQXJ035)

摘　　要：Objective: To explore how arylamine N-acetyltransferases (NATs) is related to cell apoptosis. Methods: NAT activity in apoptotic HepG2 cells was measured using high performance liquid chromatography (HPLC); the apoptosis rate of HepG2 cells acted upon by an NAT inhibitor was measured using flow cytometry. Results: NAT activity was lowered in apoptotic HepG2 cells; apoptosis rate induced by camptothecin (CAM) increased after inhibition of NAT activity in HepG2 cells. Conclusion: NAT can inhibit apoptosis in HepG2 cells.Objective： To explore how arylamine N-acetyltransferases （NATs） is related to cell apoptosis. Methods： NAT activity in apoptotic HepG2 cells was measured using high performance liquid chromatography （HPLC）; the apoptosis rate of HepG2 cells acted upon by an NAT inhibitor was measured using flow cytometry. Results： NAT activity was lowered in apoptotic HepG2 cells; apoptosis rate induced by camptothecin （CAM） increased after inhibition of NAT activity in HepG2 cells. Conclusion： NAT can inhibit apoptosis in HepG2 cells.

关 键 词：HEPG2细胞 细胞凋亡 抑制剂 生物活性 

分 类 号：R735.7[医药卫生—肿瘤]