20℃EGSB反应器中颗粒污泥的微生物种群结构分析  

Microbial Community in Granules from an EGSB Reactor Operated at 20 ℃

作　　者：邢薇[1] 左剑恶[1] 林甲[1] 李津[1] 

机构地区：[1]清华大学环境科学与工程系,北京100084

出　　处：《环境科学》2008年第9期2558-2563,共6页Environmental Science

基　　金：国家自然科学基金项目(50678091)

摘　　要：利用PCR-DGGE和RTQ-PCR等技术,对6个取自20℃下小试EGSB反应器中不同有机负荷下的颗粒污泥进行研究.结果表明,在20℃下反应器有机负荷从1.5 kg/(m3.d)逐渐提升至10.0 kg/(m3.d)的过程中,未对其污泥中古菌和真细菌的种群结构产生明显影响,各污泥样品中古菌种群相似性较高,其多样性指数分别为5.51、5.88、5.47、5.25、5.32和5.11;除接种污泥外,其余5个污泥样品中真细菌种群相似性也较高,其多样性指数分别为2.97、5.07、5.44、6.38、6.66和5.21;6个污泥样品中主要优势古菌为Methanobacterium、Methanocorpusculum、Methanosaeta和Methanospirillum等,随着有机负荷的增加和运行时间的延长,与氢营养型产甲烷菌Methanocorpusculum parvum和Methanospirillum hungatei相似的菌种的优势地位明显增强;各污泥样品中古菌的相对数量先略有降低而后逐渐升高,最终明显超过接种时的水平.The microbial communities in 6 granular sludge samples taken from a lab-scale EGSB reactor operated under different organic loading rates （OLR） at 20℃ were studied using PCR-DGGE and RTQ-PCR technique. The results indicate that as the OLR increased from 1.5 kg/（m^3·d） to 10.0 kg/（m^3·d）, the microbial communities of archaea and eubacteria in the 6 sludge samples are not changed greatly. The archaea population is highly similar among the 6 samples. The Shannon indexes of archaea population are 5.51, 5.88, 5.47, 5.25, 5.32 and 5.11, respectively. Except the seed sludge, the eubacteria population is similar among the other 5 samples. The Shannon indexes of eubacteria population are 2.97, 5.07, 5.44, 6.38, 6.66 and 5.21, respectively. The dominant archaea in the 6 granular samples are Methanobacterium, Methanocorpusculum, Methanosaeta and MethanospiriUum. As the OLR of the reactor increased and the operation time elapsed, Methanocorpusculum parvum and MethanospiriUum hungatei, both are hydrotrophic methanogens, become the dominant archaea. The archaea content in the samples decreases slightly at the beginning, but increases later and finally reaches to a level obviously higher than that in the seed sludge.

关 键 词：低温厌氧处理 颗粒污泥 微生物种群 PCR-DGGE RTQ-PCR 

分 类 号：X172[环境科学与工程—环境科学]