武夷菌素对茶树叶枯病菌(Colletotrichum camelliae)生长发育的影响  

Effects of Wuyiencin on Growth and Sporulation of Colletotrichum camelliae Causing Tea Leaf Blight

作　　者：李新[1] 王志坤[1] 谭万忠[1] 张克诚[2] 李培[1] 汪志伟[1] 殷利利[1] 谭舒心[1] 

机构地区：[1]西南大学植物保护学院,重庆400715 [2]中国农业科学院植物保护研究所,北京100094

出　　处：《西南师范大学学报（自然科学版）》2008年第3期42-47,共6页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：科技部农业科技成果转化资助项目05EFN216900354

摘　　要：茶叶枯病是茶树上最重要病害之一.该研究探讨武夷菌素对茶叶枯病菌生长发育的影响.显微观察发现。用武夷菌素处理病菌的分生孢子后,其萌发显著缓慢,萌发出的菌丝形态畸形且伸长受阻.在含有不同稀释浓度(8.3～25.0mg/L)武夷菌素的PDA培养基上,随着药液浓度的升高,病菌菌落生长受到抑制加重,单位菌落的产孢量明显降低,且产生的分生孢子不同程度地畸形.药剂对病菌的抑制效果与药液浓度成正相关.用加入武夷菌素的培养液培养病菌7d后,培养液的相对电导率显著升高(p<0.05),这说明药剂能破坏液体中病菌菌丝的细胞膜结构和通透性.Tea leaf blight caused by Colletotrichum camelliae Massee （Teleomorph： Guignardia camelliae （Cooke） Butler） is one of the most widespread and important diseases on tea crops. In this study, the effects of Wuyiencin （a mycofungicide produced from the fungus Streptornyces ahygroscopicus var. wuyiensis） on the growth, sporulation and infectivity of the pathogen C. carnelliae were examined. After the conidia were treated with Wuyiencin, the germination rates of the spores were significantly lowered; the hyphae from the germinated spores became malformed and hyphal growth was seriously hindered. On PDA plate containing different concentrations （8. 3- 25.0 mg/L） of Wuyiencin, the growth of the pathogen colonies was severely inhibited and the efficacy of inhibition was positively and linearly correlated with the concentrations of Wuyiencin. After the pathogen was cultivated with PD medium （PDA without agar） for seven days, the relative electro-conductivity of the culture liquid was increased significantly （p〈 0.05）. This implied that the compoumd interrupted the structure and function （i. e. osmosis） of haphal cell membranes of the pathogenic fungus.

关 键 词：武夷菌素 浓度 茶树叶枯病菌 菌丝及菌落生长 产孢和孢子萌发 

分 类 号：S435.711[农业科学—农业昆虫与害虫防治]