BSA-PLGA缓释微球的制备及优化条件的探索  

Explore of preparation and formulation parameters on BSA-PLGA microspheres

作　　者：黄婉丹[1] 龙大宏[1] 谷海刚[1] 

机构地区：[1]广州医学院人体解剖学教研室,广东广州510182

出　　处：《解剖学研究》2008年第4期241-244,共4页Anatomy Research

基　　金：广东省自然科学基金(06022674)

摘　　要：目的以牛血清白蛋白(BSA)为模型蛋白、聚乳酸-聚乙二醇酸(PLGA)为包裹材料,探索微球的制备方法并优化制备工艺。方法采用复乳-溶剂挥发法制备BSA-PLGA微球,显微测量微球粒径,以微量BCA法测定微球的蛋白含量并计算包封率,进行体外释放,测定微球的累积释放量。探索BSA投药量、PLGA用量、PVA浓度、超声功率等因素对微球包封率、突释量的影响。结果通过正交实验设计,优化了微球的制备工艺,其优化条件是BSA投药量为10mg、PLGA用量为250mg、PVA浓度为1.5%、超声乳化功率为60周。结论通过控制不同的因素,可以得到较高包封率、较小突释、适当载药量和粒径的BSA-PLGA微球。Objective To explore the preparation method of microsphere, optimize the preparation techniques and prescription composition. BSA was used as model protein, PLGA was used as encapsulation material to prepare microspheres. Methods Microspheres were prepared using a （water-in-oil）-in-water double emulsion solvent evaporation technique. The particle size of microspheres was measured and the encapsulation efficiency and burst release of the microspheres were calculatcd. The concentration of BSA was determined by micro BCA assay. And through these, the effects of the concentration of BSA, the amount of PLGA, the concentration of PVA and the power of ultrasonication on the particle size, encapsulation efficiency and burst release of microspheres were studied. Results Through orthogonal test, the preparation techniques of microspheres were optimized. The optimization parameters are 10 mg for BSA, 250 mg for PLGA, 1.5% PVA and 60w for uhrasonicatin power. Conclusion Smooth spherical microspheres with relatively high encapsulation efficiency, low burst release amount, proper particle size and proper drug loading amount could be produced by controlling different process parameter.

关 键 词：聚乳酸-聚乙二醇酸 牛血清白蛋白 微球 

分 类 号：R749.16[医药卫生—神经病学与精神病学] R94[医药卫生—临床医学] R318.08