大鼠全脑缺血再灌注后耳蜗组织内TrkA和NGF表达的改变  

Expression change of TrkA and NGF in cochleas of rats after whole cerebral ischemia-reperfusion

作　　者：施宁华[1] 张志坚[2] 许燕[2] 王东林[2] 周志强[2] 

机构地区：[1]江苏大学医学技术学院医学影像系,江苏镇江212001 [2]江苏大学医学院基础与临床医学研究中心,江苏镇江212013

出　　处：《基础医学与临床》2008年第8期816-819,共4页Basic and Clinical Medicine

基　　金：江苏省教育厅自然科学基金(94170)

摘　　要：目的探讨大鼠全脑缺血再灌注后酪氨酸激酶A(TrkA)和神经生长因子(NGF)在耳蜗Corti器的表达。方法建立大鼠全脑缺血再灌注模型,采用免疫组织化学染色方法检测TrkA和NGF在耳蜗中的表达;用TUNEL法检测耳蜗毛细胞发生凋亡的时相和分布。结果再灌注后6hNGF表达增强,24h达高峰后逐渐回复,再灌注24h^7dTrkA表达减弱甚至不表达,3~7d期间耳蜗毛细胞出现凋亡,7~14dTrkA表达逐渐恢复。结论TrkA的表达对于介导NGF促感觉神经细胞存活及损伤修复的生物效应有重要意义。Objective To study the expressions of tyrosine kinase A （TrkA） and nerve growth factor （NGF） on cochlear corti in rats after whole cerebral ischemia-reperfusion. Methods Establishing a model of whole cerebral ischemia-reperfusion in rats ; examining the expressions of TrkA and NGF on cochleas assuming the method of immunohistochemistry stain; and examining the time and distribution of the cochlear hair cell apoptosis through the terminal deoxynucleotidyl transferasemediated dUTP nick end labeling （TUNEL）. Results After ischemia-reperfusion the 6 h NGF expression increased, the 24 h NGF expression reached the highest and then restored gradually. After ischemia-reperfusion the 24 h - 7 d TrkA expression weakened or even didn＇ t expresse. During 3 - 7 d the cochlear hair cells presented with apoptoses. During 7 - 14 d TrkA expression restroed gradually. Conclusion TrkA expression mediats NGF to promote the sensory nerve cell surviving and for the biological effects of the repair of injury.

关 键 词：酪氨酸激酶A 神经生长因子 脑缺血再灌注 耳蜗 凋亡 

分 类 号：R743[医药卫生—神经病学与精神病学]