P27^(kip1)与出核因子CRM1在人淋巴瘤细胞系中的表达及相互关系  

Expression and relationship of P27^(kip1) with nuclear export factor CRM1 in human Lymphoma cell lines

作　　者：王燏婵[1] 赵玥铭[1] 沈爱国[1] 张冬梅[1] 黄晓东[1] 陆建新[1] 何松[2] 程纯[1] 

机构地区：[1]南通大学启秀校区微生物与免疫学教研室 [2]南通市肿瘤医院,江苏南通226001

出　　处：《基础医学与临床》2008年第8期820-824,共5页Basic and Clinical Medicine

基　　金：江苏省高校自然科学研究项目(04KJB320114);江苏省科技指导性计划(BS2004526);江苏省卫生科研项目(H200632)

摘　　要：目的研究P27kip1与出核因子CRM1在淋巴瘤细胞中的表达及相互关系。方法运用血清饥饿同步化处理淋巴瘤细胞株Jurkat和Raji,10%血清刺激细胞增殖。Western blot分别检测生长阻滞及增殖的淋巴瘤细胞中P27kip1与CRM1的表达。用核浆分离方法检测P27kip1与CRM1分别在胞核、胞质的定位。免疫沉淀检测P27kip1与CRM1在淋巴瘤细胞中的结合。结果在不同状态的淋巴瘤细胞中,P27kip1与CRM1蛋白水平的表达均表现为负相关,并且P27kip1的核内外分布与CRM1的核内外分布一致。免疫沉淀结果验证在淋巴瘤细胞中P27kip1与CRM1相互结合。结论出核因子CRM1可能通过与P27kip1结合而影响P27kip1的核内外分布及表达水平,从而参与调控淋巴瘤细胞的生长。Objective To investigate the expression and relationship of P27^kip1 with nuclear export factor CRM1 in lymphoma cells. Methods Cells were synchronized by serum starvation, and 10% serum stimulating proliferation. The expression of P27^kip1 and CRM1 was detected by cell fractionation and Western blot. Results The albumen amount of P27^kip1 increased while albumen amount of CRM1 was decreased. The reverse changes happened after serum release. The location of P27^kip1 was accorded with the expression of CRM1. P27^kip1 and CRM1 could form compound in Jurkat and Raji cells as detected by immunopreeipitation. Conclusion CRMI may influence the location and expression of P27^kip1 through integrating with P27^kip1, and then may participate in regulating the growth of lymphoma cells.

关 键 词：P27^KIP1 CRN1 出核 淋巴瘤 

分 类 号：R733.1[医药卫生—肿瘤]