激活蛋白-1与p53基因启动子结合的染色质免疫沉淀技术分析  

Preliminary study of p53 gene promoter binding site of activator protein-1 in A549 cell lines by chromatin immunoprecitation

作　　者：沈飞[1] 李智涛[1] 吴逸明[1] 吴卫东[1] 

机构地区：[1]郑州大学公共卫生学院职业卫生与职业医学教研室,河南450001

出　　处：《中华劳动卫生职业病杂志》2008年第8期453-456,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目（30571552）

摘　　要：目的 检测肺腺癌A549细胞内的转录因子激活蛋白-1（AP-1）与p53基因调控区的结合情况。方法采用染色质免疫沉淀技术，用c-jun特异性抗体沉淀DNA，聚合酶链反应（PCR）技术检测p53基因5’端特异性序列。结果在c-jun特异性抗体免疫沉淀的DNA片段中扩增出p535’端的特异性序列。结论AP-1在A549细胞内结合于p53基因的调控区，参与该基因的表达调控。Objective To detect and verify activator protein-1 （,AP-1） binding to p53 gene promoter region in A549 cell lines in vitro. Methods AP-1 binding to p53 gene promoter region was detected by chromatin immunoprecipitation （CHIP） assay using c-jun specific antibody, and PCR amplified its gene specific DNA fragment. Results The p53 gene specific fragment was found in the DNA fragment immunoprecipitated by c-jun antibody, Conclusion AP-1 binds to p53 gene promoter region in A549 cells,and regulates its expression,

关 键 词：转录因子AP-1 基因 p53 沉淀素试验 

分 类 号：R734.2[医药卫生—肿瘤] R563[医药卫生—临床医学]