mag-1基因与肿瘤细胞转移的相关性研究  

Investigation of the association between mag-1 gene and metastasis

作　　者：贾海泉[1] 蔺会云[1] 徐元基[1] 杜芝燕[1] 王妍[1] 陈惠华[1] 姜云波[1] 陆应麟[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850

出　　处：《军事医学科学院院刊》2008年第4期335-339,352,共6页Bulletin of the Academy of Military Medical Sciences

基　　金：国家自然科学基金资助项目(30271452;30570800);国家"973"计划资助项目(2002CB513105)

摘　　要：目的:探讨mag-1基因与肿瘤细胞转移的关系。方法:检测mag-1在人肺巨细胞癌高低转移细胞株PLA801D与PLA801C、肺癌高低转移细胞株A549与H1299及乳腺癌高低转移细胞株MCF-7与MDA-MB-231中mag-1的表达水平;检测构建的mag-1正反义真核表达载体转染细胞后mag-1表达水平的变化;将mag-1正义表达载体分别转染低转移细胞株PLA801C及MCF-7并筛选出稳定克隆;研究mag-1过表达对细胞形态、增殖、迁移、黏附及侵袭等生物学行为的影响。结果:高转移细胞株PLA801D中mag-1的mRNA及蛋白表达水平均显著高于低转移细胞株PLA801C中mag-1的表达水平,乳腺癌高低转移细胞株中mag-1的蛋白表达水平也有明显的差异;过表达mag-1细胞的形态及增殖能力没有发生改变,而细胞的黏附、迁移及侵袭能力增强。结论:mag-1可能促进肿瘤细胞的转移。Objective：To investigate the association between mag-1 gene and metastasis. Methods： The differential expression of mag-1 between highly（PLA801D） and poorly（PLA801C） metastatic sublines of human lung giant cell carcinoma was determined. The expression of mag-1 in another pair of lung cancer cell lines（A549/H1299） and breast cancer cell lines（MDA-MB-231/MCF-7） with different invasive activity was also determined. The effect of the sense and the antisense expression vectors of mag-1 on the expression of mag-1 was examined after they were transiently transfected separately into PLA801C and PLAS01D. The poorly metastatic cells PLA801C and MCF-7 were stably transfected with sense expression vector of mag-1 and the morphology, proliferation, migration, adhesion and invasion of cells with overexpressing mag-1 were assayed. Results： PLA801D And MDA-MB-231, which had strong metastatic activity, expressed mag-1 more abundantly than PLA801C and MCF-7 cells,which had low metastatic activity. The morphology and the proliferation of PLA801C and MCF-7 overexpressing mag-1 did not change, but their ability of migration, adhesion and invasion increased as compared with cells transfected with control vector. Conelusion：mag-1 Might promote the metastasis of cancer cells.

关 键 词：mag-1 肿瘤转移 转染 基因表达 肺肿瘤 乳腺肿瘤 

分 类 号：R730.2[医药卫生—肿瘤]