大鼠海马突触体素及胆碱乙酰转移酶的增龄变化  

Changes of hippocampal synaptophysin and choline acetyltransferase in age-increasing rats

作　　者：王蓉[1] 赵志炜[1] 唐玉[2] 张丽[3] 张兰[3] 李林[3] 

机构地区：[1]首都医科大学宣武医院中心实验室 [2]首都医科大学药理学系 [3]首都医科大学宣武医院药物研究室教育部神经变性病学重点实验室,北京100053

出　　处：《中国老年学杂志》2008年第16期1571-1573,共3页Chinese Journal of Gerontology

基　　金：国家重点基础研究计划-973计划项目(2003CB517104);国家自然科学基金项目(90709011;30472184);北京市自然科学基金项目(7032013)

摘　　要：目的观察不同月龄（1、3、6、12、18、24月龄）大鼠脑海马区突触体素（SYP）和胆碱乙酰转移酶（ChAT）表达的变化,探讨其与学习记忆功能之间的关系。方法采用免疫组织化学染色方法,观察SYP和ChAT在增龄大鼠海马中的表达水平。结果1-6月龄SYP免疫反应阳性产物逐渐增加,6-18月龄缓慢减少,进入24月龄明显减少,与其他各组相比具有非常显著性差异（P〈0.01）。ChAT免疫反应阳性神经元在1月龄时比较少,3-6月龄时最多,以后明显减少,进入24月龄后,偶见阳性细胞。结论SYP和ChAT在大鼠海马中的表达存在随龄变化,特别是老年期表达量显著降低,可能是导致学习记忆障碍的重要机制之一。Objective To investigate the changes of hippocampal synaptophysin （SYP） and choline acetyltransferase （CHAT） in age-increasing rats （ 1,3,6,12,18 and 24 months）, and evaluate the correlation with learning-memory abilities. Methods The expression of SYP in whole hippocampus and ChAT in CA1 area was measured by immunohistochemistry. Results Under the light microscope,SYP immunoreaction products located in all regions of hippocampus, mainly in the form of panicles and dot sands. The expression of SYP was low at the age of 1 month,increased gradually,reached the peak in 6-month-old, then decreased gradually. But from 3 to 18-month-old, there were not significant difference among different groups. The expression of SYP decreased markedly at 24 months of age, and had significant difference compared with other groups （P 〈0.01 ）. ChAT immunoreaction neurons in CA1 area were few at the age of 1 month ,most and staining darkly in 3 and 6-month-old,then decreased markedly. There were rare ChAT immunoreaction neurons at the age of 24 months. Conclusions The expression of hippocampal SYP and ChAT shows changes with aging,decreases in aged rats especially,which may be one of the mechanisms involved in learning-memory deficit.

关 键 词：增龄 突触体素 胆碱乙酰转移酶 海马 大鼠 

分 类 号：R-332[医药卫生] R338.13