吡啶降解菌的筛选及作用机理研究  

Screening of Pyridine Degrading Strains and the Study of Their Degradation Mechanism

作　　者：李叶珺[1] 李文英[1] 徐英[1] 冯杰[1] 

机构地区：[1]太原理工大学煤科学与技术教育部和山西省重点实验室,山西太原030024

出　　处：《太原理工大学学报》2008年第5期490-494,共5页Journal of Taiyuan University of Technology

基　　金：教育部创新团队发展计划(IRT0517);山西省自然科学基金资助项目(20051017);山西省回国留学人员基金资助项目(2005-21)

摘　　要：煤中有机氮主要以吡啶、吡咯结构形式存在。为了探索微生物对煤中有机氮的脱除效果，采用平板固体筛选和液体筛选相结合的方法，以模型化合物吡啶作为氮源对降解菌进行筛选，得到吡啶降解能力较高的JH-2菌株。通过分析影响菌种降解能力的因素，如培养基种类、接种量、pH值及温度等，获得JH-2菌株降解吡啶的最佳实验条件：以葡萄糖为碳源，温度35～40℃，pH值为7。在此条件下，吡啶降解率可达到40．9％。对JH-2菌株降解吡啶机理的初步研究表明，吡啶中的氮在降解酶的作用下转化为NH4^＋离子，并作为微生物生长代谢过程中的氮源被消耗利用。The microorganisms which could degrade pyridine were developed and trained in this article. Pyridine was selected as a unique nitrogen source for screening the microbes. A strain JH-2 was screened out from the activated sludge of a coke plant and identified as staphylococcus. The optimum culture medium and culture conditions of the strain JH-2 were studied with single factor test. Results showed that the strain JH-2 was easy to cultivate, and the optimized degradation conditions were 35-40℃ at a pH value of 7. The highest degradation rate of pyridine is 40.9% after 48h. Degradation mechanism of pyridine by the strain JH-2 was suggested that nitrogen in pyridine was changed into NH4 ^＋ by enzyme and then was used up during metabolism.

关 键 词：吡啶 微生物降解 菌种 降解机理 

分 类 号：X172[环境科学与工程—环境科学]